Limnophila aromatica在東南亞常被用作香料及藥草。本研究使用水及不同濃度(50%, 75% 及100%)之甲醇、乙醇及丙酮水溶液為溶劑來萃取 L. aromatica. 利用各種體外測定法量測經冷凍乾燥L. aromatica萃取液之抗氧化活性、總酚含量及總黃酮化合物含量。以100% 乙醇所得萃取液顯示有最高之抗氧化活性、最大還原能力及DPPH (2,2-diphenyl-1-picrylhydrazyl)自由基清除活性。同一萃取液亦顯示有最高之總酚含量(40.5 mg gallic acid equivalent/g of defatted L. aromatica)及總黃酮化合物含量(31.11 mg quercetin equivalent/g of defatted L. aromatica)。以50%丙酮水溶液萃取時可得到最大之萃取產率。這些結果顯示 L. aromatica有降低氧化壓力之潛力，可應用於食物添加。
本研究利用濕式研磨法從L. aromatica中分離澱粉。分離得到之澱粉純度為 84.76 %,而 71.12% 之總澱粉為抗性澱粉。澱粉中直鍊澱粉之含量為1.19 %。澱粉顆粒之直徑大小約 6.22 到110 m。本研究也測定所得澱粉之物化性質如膨潤力、粘性、糊化、回復及分解溫度。研究結果顯示從去脂L. aromatica分離所得之澱粉具有優良之物化性質，是以可成為食品，藥物及高分子工業之原料；且有潛力與目前所使用之原料如小麥與玉米競爭。藉由管柱層析可從乙酸乙酯及甲醇分率中分離出三種酚化合物。這些酚化合物之結構可利用光譜法(MS, IR, NMR, UV-Vis)求得，其中有二者為之前未報告之化合物。

Limnophila aromatica is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatic extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatica) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatica). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.
In this study, starch was isolated from L. aromatica by using wet-milling method. The purity of the starch product was 84.76 % and 71.12% of the total starch was the resistant starch. The amylose content of the starch was 1.19 %. Starch particle diameters were found to be 6.22 to 110 m. Physicochemical properties such as swelling power, pasting, gelatinization, retrogradation and decomposition temperature were determined. The results indicate that starch from defatted L. aromatica possesses good physicochemical properties and can be considered as a prospective renewable raw material in food, pharmaceutical and polymer industries with potential to compete with current feedstock such as wheat and maize.
Three phenolic compounds were isolated from ethyl acetate fraction and methanol fraction of L. aromatica by using low-pressure column chromatography. Their structrures were identified by spectral methods (MS, IR, NMR, UV-Vis). Two novel compounds, (E)-methyl-3-(3-hydroxy-4-(4-hydroxy-2-(5,6,7,8-tetrahydroxy-3-(4-isopropoxybutan-2-yloxy)-4-oxo-4H-chromen-2-yl)phenoxy)phenyl)acrylate and (E)-methyl-3-(3-hydroxy-4-(4-(3-methoxy-1-(3,4,5-trihydroxy-6-((3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yl)methoxy)tetrahydro-2H-pyran-2-yl)propoxy)-2-(3,5,6,7,8-pentahydroxy-4-oxo-4H-chromen-2-yl) phenoxy) phenyl) acrylate were identified.

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