其中一个不能很好地识别药用植物是萨朗semut（Myrmecodia决案件），这可能是治疗agents.Traditionally的新来源，人们通常会用巨大的肿胀（称为块茎）萨朗semut作为草药的一部分。萨朗针对不同的癌细胞semut的提取物的功效进行了报道。所观察到的抗氧化活性和萨朗semut提取的治疗效果被认为是由于包含在它的抗氧化剂化合物。

使用水浴萃取在55℃从萨朗semut抗氧化剂的回收进行了研究。的参数，如提取时间的影响，溶剂混合物和溶剂采样比的组合物进行了研究，在三个级别的每个和最佳提取参数为：时间，4小时;溶剂（乙醇/水;体积/体积），80％;和溶剂而采样率，50毫升/克。在这些最佳条件，得到的13.82％的产率。利用DPPH自由基的提取物的自由基清除活性（抗氧化活性）进行评价的IC50发生在96.21±9.03μg/mL的提取物。总酚含量（TPC）和总黄酮含量（TFC）采用的设计方法确定，发现分别为330.61±2.13毫克没食子酸当量/克干提取物和63.28±1.75毫克槲皮素当量/克干提取物。在最佳条件下得到的提取物用HPLC分析和5黄酮类化合物被识别和它们的内容是（毫克/克干提取物）：山奈酚（13.767，luteoline（0.005），rutine（0.003），槲皮素（0.030），芹菜素（4.700）。

作为溶剂;用热回流萃取法，用乙醇/水（体积/体积80:20）萨朗semut萃取。使用己烷和乙酸乙酯的粗提取物（CE）进行分级分离。乙酸乙酯部分（EAF）被选定为根据它的抗氧化活性进一步分析。两个主要的酚类化合物（迷迭香酸和原花青素B1）进行了鉴定，确认和使用的技术，LC / ESI/ MS / MS，UV / Vis和高效液相色谱组合进行定量。但确认第三化合物（原花青素B1的低聚物）和定量受阻于缺乏商业标准。的迷迭香酸和原花青素B1中的浓度被发现是，分别20.688±1.573毫克/克干样品和3.236±0.280毫克/克干样品。 

可靠的RP/HPLC/UV分析方法估算总酚和总黄酮含量的开发。商业没食子酸，抗坏血酸，槲皮素和儿茶素用作方法开发期间的标准。总酚含量（TPC）和总黄酮含量（TFC）从萨朗的甲醇提取物来确定semut采用高效液相色谱法。在这项工作中所开发的高效液相色谱法，通过分析加标样品的公知的分光光度法进行比较。像标准偏差，平均值，百分比回收率和％相对标准偏差（％RSD）的参数被用来比较这两种方法。精度（％回收）的新方法，从90.82％到101.34％和％相对标准偏差小于1.0％。这些值是通过使用分光光度测定方法获得的恢复％和％RSD的范围内（89.44 - 101.90恢复％和0.44 - 3.59％RSD）。

One of the not well identified medicinal plants is sarang semut (Myrmecodia pendens) which might be a new source of therapeutic agents.Traditionally, folks usually use the huge swelling (known as tuber) part of sarang semut as herbal remedies. The efficacy of extracts of Sarang semut against different cancer cells was reported. The observed antioxidant activity and therapeutic effect of sarang semut extract was supposed to be due to antioxidant compounds contained in it.
The recovery of antioxidants was investigated using water bath extraction at 55oC from sarang semut. The effects of parameters such as extraction time, composition of solvent mixture and solvent to sample ratio were investigated at three levels for each and the optimum extracting parameters were: time, 4 h; solvent (ethanol/water; v/v), 80%; and solvent to sample ratio, 50 mL/g. Under these optimal conditions, a yield of 13.82% was obtained. The free radical scavenging activity (antioxidant activity) of the extract was evaluated using DPPH radical and the IC50 occurred at 96.21 ± 9.03 μg /mL of extract. The total phenol content (TPC) and total flavonoid content (TFC) were determined using the designed methods and found to be 330.61 ± 2.13 mg gallic acid equivalent/g dry extract and 63.28 ± 1.75 mg quercetin equivalent/g dry extract respectively. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds are identified and their contents are (mg/g dry extract): kaempferol (13.767, luteoline (0.005), rutine (0.003), quercetin (0.030) and apigenin (4.700).
The crude extract (CE) obtained under optimum condition was fractionated using hexane and ethyl acetate. Ethyl acetate fraction (EAF) was selected for further analysis based on its antioxidant activity. Two major phenolic compounds (rosmarinic acid and procyanidin B1) were identified, confirmed and quantified using a combination of techniques LC/ESI/MS/MS, UV/Vis and HPLC. But confirmation and quantification of the third compound (oligomer of procyanidin B1) was hampered by lack of commercial standard. The concentration of rosmarinic acid and procyanidin B1 was found to be 20.688 ± 1.573 mg/g dry sample and 3.236 ± 0.280 mg/g dry sample, respectively.
Reliable RP-HPLC/UV analytical method was developed for estimation of total phenolic and total flavonoid contents. Commercial gallic acid, ascorbic acid, quercetin and catechin were used as standards during method development. Total phenolic content (TPC) and total flavonoid content (TFC) were determined from methanol extracts of sarang semut using HPLC method. The HPLC method developed in this work was compared with the well-known spectrophotometric method by analyzing spiked samples. Parameters like standard deviation, mean value, percentage recovery and % relative standard deviation (%RSD) were used to compare the two methods. The accuracy (% recovery) of the new method varies from 90.82% to 101.34 % and %RSD was less than 1.0%. These values are within the range of % recovery and % RSD obtained by using spectrophotometric method (89.44 - 101.90 %recovery and 0.44 – 3.59 %RSD).

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