本研究旨在開發明膠海綿敷料應用於醫療器材領域，以明膠為基底，透過發泡與冷凍乾燥的方式製備成敷料，此敷料提供良好的孔隙分佈，平均孔徑約為60~70 μm左右，可幫助吸收傷口滲出液，促進傷口恢復；並對3T3、L929、Hs68、CG1519四株細胞株進行細胞存活率分析，明膠海綿敷料釋出液對四株細胞株皆表現出良好的生物相容性。動物實驗方面，以BALB/cByJNarl品系的小鼠進行創傷癒合研究，對傷口癒合具有促進皮膚功能性組織修復再生的效果更換敷料時進行的傷口影像面積紀錄，在創傷部位癒合收口方面表現出與市售敷料相當的復原速度，明膠海綿敷料的組別有較穩定的收口速率表現。於病理切片中可觀察到傷口床的位置，具有再生的組織與表皮，表現了在傷口癒合的功效性，在肝腎切片中沒有發現中性球的存在，也沒有不正常的淋巴球瀰漫現象發生，顯示此敷料由細胞存活率與動物組織切片中的結果對實驗小鼠不具有顯著藥物毒性，證實此明膠海棉敷料對生物體具有良好的安全性，本研究成功開發明膠海綿結構於傷口敷料應用。

The aim of this study was to develop a gelatin-based sponge dressing for application in the medical device field. Gelatin was used as the substrate, and the dressing was prepared through foaming and freeze-drying processes. This dressing exhibited favorable pore distribution, with an average pore size of approximately 60-70 μm, enabling efficient absorption of wound exudate and promoting wound healing. Cell viability analyses were conducted on four cell lines (3T3, L929, Hs68, CG1519) exposed to the released fluids from the gelatin sponge dressing, demonstrating excellent
biocompatibility across all cell lines.
In animal experiments, wound healing studies were carried out on BALB/cByJNarl mice. The gelatin sponge dressing exhibited a pronounced effect in promoting functional tissue regeneration and skin repair at the wound site. Comparative wound closure rate analysis, based on recorded wound area images during dressing changes, revealed a recovery speed comparable to that of commercially available dressings. The gelatin sponge dressing group consistently demonstrated a stable wound closure rate. Pathological sections of the wounds displayed regenerative tissues and epidermal layers, highlighting its wound healing efficacy. Liver and kidney sections showed no presence of neutrophils and lacked abnormal lymphocyte infiltration, indicating the absence of significant drug toxicity in experimental mice based on cell
viability and tissue slice results.
These findings collectively demonstrate the favorable safety profile of the gelatin sponge dressing within biological systems. In conclusion, this study successfully developed a gelatin sponge structure for wound dressing applications.

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