本論文係將分別含有幾丁聚醣(chitosan, CS)及聚乳酸(polylactic acid, PLA)之溶液以不同比例混合，經由電紡法製成奈米纖維。特別的是，該紡液的溶劑組成為氯仿、乙醇、乙酸、水。所得奈米纖維的形態係以SEM檢視之，而細胞毒性則以纖維組織母細胞評估之。幾丁聚醣在奈米纖維中的存在則以染色法及光譜儀測定之。該奈米纖維之抗菌性則以大腸桿菌檢測。SEM影像顯示CS-PLA奈米纖維的直徑係在奈米等級。而隨著CS的比例增加，珠狀物的數目亦隨之增加。酸性染料Orange II的染著證實該CS-PLA奈米纖維中含有幾丁聚醣。另外L929纖維組織母細胞在CS-PLA奈米纖維上生長率良好，顯示無生物毒性。此外，CS-PLA具有對大腸桿菌(E. coli)之抗菌性。由於CS-PLA奈米纖維具有高抗菌性及生物相容性，顯示CS-PLA具有作為創傷敷材的潛力。

In this thesis, nanofibers of poly(lactic acid) (PLA) blended with chitosan (CS) were fabricated via the electrospinning from solutions containing CS and PLA in various ratios. In particular, the solvent of the solution was a mixture of chloroform, ethanol, acetic acid and water. The morphological characteristics of nanofibers was examined using scanning electron microscope (SEM). The cytotoxicity of nanofibers was evaluated based on the proliferation of fibroblasts. The presence of chitosan in CS-PLA nanofibers were characterized using UV-Visible spectrophotometer. The antibacterial activity of these CS-PLA nanofibers was determined against Escherichia coli (E. coli).
SEM images showed that average diameter of Chitosan-PLA nanofibers were nanometer length of scale. In addition, the number of beads increased with the increase of chitosan content in the Chitosan-PLA nanofibers. The uptake of an acidic dye (orange II sodium salt) indicated the presence of chitosan in the nanofibers. By culturing with L929 fibroblasts, CS-PLA nanofibers showed good cell viability, indicating non-cytotoxicity. Furthermore, CS-PLA nanofibers exhibited antibacterial activity against E. coli.
The high antibacterial activity and biocompatibility suggests that these CS-PLA nanofibers have a potential in biomedical fields, especially for wound dressing applications.

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