本研究利用原地還原法製備奈米銀摻合之聚麩胺酸/聚乙烯醇複合水膠膜。在此膜中，硝酸銀為聚乙烯醇還原，並用聚麩胺酸作穩定劑。分別觀察所形成奈米粒子之大小、分佈情形及其拉曼表面增強效應（SERS），並且探討其血液相容性及生物相容性。
根據FE-SEM之測量，奈米複合膜表面具有均勻分散之奈米銀粒子，其平均粒徑90 nm，且隨著PGA的含量增加而粒徑減小，亦可提高複合水膠膜之膨潤度，及減少銀離子析出量。而蛋白質及血小板的吸收方面會隨著PGA的增加而下降，而延長複合膜之凝血時間。本實驗中以抗藥性金黃色葡萄球菌及綠膿桿菌檢測出奈米複合水膠膜在銀含量為10-5M即具抗菌性，也以纖維母細胞L929進行細胞毒性試驗證明複合水膠膜不具細胞毒性。此膜對於苯甲酸之SERS強化係數約為106 。
綜觀所有結果，顯示PGA/PVA/Ag之複合水膠膜，其抗凝血性、生物相容性及應用在生醫材料上相當有潛力，且其高SERS效應未來可應用於生化分析所需的超高靈敏性偵測。。

An in-situ reduction method was employed to prepare hemocompatible and surface-enhanced Raman scattering (SERS)-active substrates. In this nanocomposite hydrogel membrane, silver nitrate was stabilized with poly(γ-glutamic acid) (γ-PGA).and reduced by polyvinyl alcohol (PVA), thereby resulting silver nanoparticles were doped in PGA/PVA composite hydrogels. Based on the results from Field emulsion scanning electron microscopy (FE-SEM) measurement, the surfaces of nanocomposite hydrogel membrane had well-dispersed spherical silver nanoparticles with an average diameter of 90 nm. It was found that the reduced protein adsorption and platelet adhesion and prolonged blood coagulation time would occur in the presence of PGA. The SERS enhancement of this film had an enhanced factor about 106 for benzoic acid. The cytocompatibility was proven safe by the in-vitro cell cultivation of L929 fibroblasts. Overall results demonstrated that the biomedical silver nanoparticle-doped PGA/PVA composite hydrogel membrane possessed excellent hydrophilicity, antibacterial activity, hemocompatibility and cytocompatibility. Besides, it would be potential as SERS-active substrate for biosensor application.

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