生物相容性之一氧化氮 (NO) 傳遞系統在抗菌上的應用近年來頗受重視。而本論文是以磁性奈米顆粒(MNP)為一氧化氮之載體，開發可利用磁場回收再填充之一氧化氮傳遞系統，利用氨基丙基三乙氧基硅烷 (APTES) 表面改性MNP，用于從表面產生樹狀聚合物poly(amidoamine) (PAMAM)，PAMAM合成到第 3 代 (G3)後與十二烷基醛和 DMAB 以Schiff-base反應產生二級胺結構。此外也利用3--缩水甘油基丙基三乙氧基硅烷（GPTES）修飾於MNP表面，再接枝 上PEI。利用MNP表面所接上之二級胺，施以 10 atm NO 以生成能釋放NO之diazeniumdiolate。所製備之NO 釋放MNP 經 FTIR、SEM、EDS 和 DLS分析，顆粒無聚集現象，粒徑約250 nm，其中MNP@PEI 1% 有最高的 NO 釋放能力 493 nM/h/mg/mL，且持續超過 3 天。MNP@PEI 1% 可以殺死 100% 的大腸桿菌和 53% 的金黃葡萄球菌。此外，殼聚糖硫聚体 (CS-SH) 和疏水改性殼聚糖 (HMCS) 也被製備用作 NO 釋放材料。硫醇殼聚糖硫聚体 CS-SH@NO 的 NO 釋放能力為 1.69 μM/h/mg/mL，以甲醇濃度 (60%, v/v) 作為 NO 充填的溶劑， HMCS@NO 之NO 釋放能力4.81 μM /h/mg/mL。 CS-SH@NO 可殺死 54% 的大腸桿菌和 36% 的金黃葡萄球菌，而HMCS@NO 可殺死約 66% 的大腸桿菌和42% 的金黃葡萄球菌。

Recently, a promising strategy for biocompatible nitric oxide (NO)-delivery systems has gained attention in therapeutic applications, especially antimicrobial applications to kill bacteria. In this study, the MNP was modified with aminopropyltriethoxysilane (APTES) for dendrimer grafting. Aminosilane-MNP was grafted with PAMAM dendrimer, and PAMAM generations were synthesized up to generation 3 (G3). Then, secondary amine N-diazeniumdiolate of PAMAM on MNP was synthesized via Schiff base reaction between amine groups of PAMAM and dodecyl aldehye and DMAB. Besides that, MNP was also modified with (3–Glycidylocypropyl)triethoxysilane (GPTES) for grafting PEI. Then, N-diazeniumdiolate of PEI was synthesized charging with 10 atm NO. The synthesized MNP NO-releasing materials were characterized by FTIR, SEM, EDS, and DLS. SEM images showed that the synthesized nanoparticle have uniform size and there is no aggregation between each particle, and the particle size diameter is ~250 nm. Compared to the other synthesized nanoparticle, MNP@PEI 1% have the highest NO release of 493 nM/h/mg/mL and last for over 3 days. The antibacterial activity was determined by using Gram negative (E. coli) and Gram positive (S. aureus). The result showed that MNP@PEI 1% could kill 100% of E. coli cells and 53% S. aureus cells. Furthermore, chitosan thiomer (CS-SH) and hydrophobically modified chitosan (HMCS) were also used as NO-releasing materials. S-nitrosothiol chitosan thiomer was synthesized via chitosan isothiouronium salt intermediate with thiourea reagent under microwave irradiation, followed with thiol nitrosation. The release rate of NO for CS-SH@NO was estimated to be 1.69 μM/h/mg/mL, and for HMCS@NO with methanol concentration (60%, v/v) as NO charging solvent was estimated to be 4.81 μM/h/mg/mL. CS-SH@NO could kill around 54% of E. coli cells and 36% of S. aureus cells, and HMCS@NO with methanol concentration (60%, v/v) could kill around 66% of E. coli cells and 42% of S. aureus cells.

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