近幾年來罹患癌症者日益增加，癌症治療方式也越來越多樣化，而透過近紅外光(NIR，808 nm)的非侵入式光熱治療法也逐漸受到關注。本研究中使用近紅外線(NIR，808 nm)，進行對癌症非侵入性的光熱治療法，運用奈米鉑的高穩定性、抗腐蝕性、於有水分的環境下可釋放電子,具有抗氧化作用及還原作用，與奈米金(Au NPs)的光學性質和良好的生物相容性結合而成之奈米鉑-金雙金屬(Pt/Au NPs)，促使Pt/Au NPs比Au NPs照射近外光線時，有更穩定良好的升溫效果。透過升溫曲線顯示Pt/Au NPs可於5分鐘內達到約58 °C ，而表現出了良好的光穩定性，光熱轉換率為55 %，與Au NPs相比以達到良好之效果。通過穿透式電子顯微鏡(Transmission electron microscope，TEM)表面特徵分析結果顯示Pt/Au NPs和Au NPs經過離心後，表面形態並未產生任何變化，仍是球狀形態，粒徑大小約為20-30 nm。MTT檢測使用有無分散劑及光熱治療前後的Pt/Au NPs和Au NPs對卵巢癌細胞SKOV-3 cell及乳腺癌細胞JC cell進行體外細胞試驗，發現Pt/Au NPs和Au NPs無分散劑時，細胞存活率可達到80 %以上，對細胞較無毒性，而光熱治療後，可有效殺死癌細胞，而Pt/Au NPs無論細胞毒性或是治療效果都比Au NPs來的好。動物實驗中，透過動物的體重及腫瘤觀察出，注射Pt/Au NPs時並未影響動物的體重及身理狀況，而光熱治療使近紅外光穿透於皮膚，使Pt/Au NPs達到升溫效果，並有效抑制腫瘤生長，透過動物器官病理組織切片可觀察出並未對動物器官造成傷害，而動物腫瘤病理組織切片發現腫瘤內部血管減少，可代表治療過程中癌細胞無法索取較多養分，而導致癌細胞死亡，使腫瘤大小縮小44.4 %。這些結果解釋了，Pt/Au NPs之光熱轉換率與細胞毒性都比Au NPs來的還要好，進行動物體內實驗，Pt/Au NPs也展現了良好的光熱治療效果，能夠有效抑制腫瘤，Pt/Au NPs的特性於未來的光熱治療中具有強大的潛力及應用。

The number of cancer patients has been increasing in recent years, and cancer treatment modalities are becoming more and more diverse. In this study, we used near-infrared (NIR, 808 nm) light to perform non-invasive photothermal therapy for cancer. By using the high stability, corrosion resistance, electron release in the presence of moisture, antioxidant and reduction effects of nano-platinum in conjunction with the optical properties and good biocompatibility of gold nanopartcles (Au NPs) to form platinum-gold bimetals nanopartcles (Pt/Au NPs). The geuerated of Pt/Au NPs and Au NPs has resulted in a more stable heating effect than that of Au NPs when exposed to near external light. The warming curve shows that Pt/Au NPs can reach about 58°C in 5 minutes and exhibit good photostability with a 55% photothermal conversion rate, which is better a compared to Au NPs any. The results of surface characterization by transmission electron microscope (TEM) showed that the surface morphology of Pt/Au NPs and Au NPs did not change after centrifugation, but remained spherical with particle size of about 20-30 nm. In an in vitro cell test on ovarian cancer cells SKOV-3 cells and breast cancer cells JC cells with and without Pt/Au NPs and Au NPs, it was found that the cell survival rate of Pt/Au NPs and Au NPs without dispersant could reach more than 80%, which was less toxic to cells, and after photothermal treatment, it could effectively kill cancer cells. Pt/Au NPs are better than Au NPs in terms of cytotoxicity and therapeutic effect. In the animal experiments, the weight and tumors of the animals were observed, and the weight and physical condition of the animals were not affected by the injection of Pt/Au NPs, while the photothermal treatment penetrated the skin with near-infrared light to achieve the warming effect of Pt/Au NPs and effectively inhibit the growth of tumors. The reduction of blood vessels inside the tumor may mean that the cancer cells could not claim more nutrients during the treatment process, resulting in the death of cancer cells and a 44.4% reduction in tumor size. These results explain that the photothermal conversion rate and cytotoxicity of Pt/Au NPs are better than those of Au NPs, and Pt/Au NPs have demonstrated good photothermal therapeutic effects in animal in vivo experiments, which can effectively inhibit tumors.

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