本研究利用靜電紡絲技術與特殊電紡方式的改良，製備出具有芯殼結構之海藻酸與幾丁聚醣複合纖維膜，接著將此薄膜透過浸潤加工使其外表包覆一層透明質酸。利用混摻過甘油的海藻酸進行靜電紡絲大大增加了電紡的可行性。另外結合幾丁聚醣及透明質酸本身於水溶液中之聚電解質特性成功製備具有三層結構之聚電解質複合材。透過物性分析、材料穩定度測試、生物相容性分析，評估其對於應用於傷口敷材之可行性。
經實驗數據發現，靜電紡絲參數的控制是成功紡出複合聚電解質薄膜的重要關鍵。經由纖維平均直徑的計算，除了可以歸納出理想電紡操作參數值，更可以證明靜電紡絲薄膜之高比表面積、高孔隙度特性。本研究以幾丁聚醣做為靜電紡絲之凝固液，薄膜成型後亦有效提升其機械性質。薄膜透過浸潤加工透明質酸後其細胞相容性有效提升，在模擬傷口癒合之細胞測試結果，本研究所製備之海藻酸-幾丁聚醣、海藻酸-幾丁聚醣-透明質酸複合膜更能有效促進細胞的遷移。

In this study, we combined electrospinning technique and the coagulating bath method to prepeare alginate (AL) and chitosan (CS) composite fibers with core-shell structure, followed by coating the resulting mats with a layer of hyaluronic acid (HA). The property of glycerol-containing AL enhanced the spinnablilty. Because of the polyelectrolytic nature of AL, CS and HA, a three-layer structure was formed. Through characterization of physical properties, stability testing, and biocompatibility, the feasibility of these layered mats for wound dressing was evaluated.
The electrospinning parameters were varied to reach the suitable for preparing AL-CS composite fibers. The average fiber diameter was used as the index for evaluating the electrospinning parameters, and exhibiting the high surface area and the high porosity of electrospun mats. The results showed that the CS can coagulate AL fiber with enhanced mechanical properties. The coating of HA can also enhance the cytocompatibility. The results of the simulated wound healing assay test showed that AL-CS and AL-CS-HA composite mats would be more effective in promoting the cell migration than conventional calcium-coagulated AL fibers.

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