研究生: |
魏廷宇 Ting-yu Wei |
---|---|
論文名稱: |
以材料染色改善光固化PCL-DA/PEG-DA支架精度之研究 Study on improving the accuracy of photo-cured PCL-DA/PEG-DA scaffold by coloring the material |
指導教授: |
鄭逸琳
Yih-lin Cheng |
口試委員: |
何明樺
Ming-Hua Ho 江偉宏 Wei-Hung Chiang |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 機械工程系 Department of Mechanical Engineering |
論文出版年: | 2016 |
畢業學年度: | 104 |
語文別: | 中文 |
論文頁數: | 123 |
中文關鍵詞: | 積層製造 、PCL 、PEG-diacrylate 、顏料 、染料 、分散劑 |
外文關鍵詞: | Additive Manufacturing, PCL, PEG-diacrylate, Pigment, Dye, Dispersant |
相關次數: | 點閱:465 下載:3 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
組織工程支架PCL-DA+PEG-DA因為透明材料有透光性,以往均會遇到光穿透導致過固化,以及使用丙酮造成的光固化支架收縮問題。而在先前研究有顯示利用支架染色的方式能改善光穿透之問題。因此本研究尋找市售的無毒性染料及顏料來取代原先的油墨染料,應用於組織工程支架的製作,並以對於組織工程支架的細胞貼附狀況為依據判別其生物相容性是否良好,其於組織工程上的應用價值。同時採以加熱至熔點的方式,使PCL-DA熔化與PEG-DA混合,取代加入丙酮混合的方式,解決丙酮於光固化材料時造成的收縮問題。且因需加熱熔化,故於原先DLP動態式光罩系統內架設一材料槽與溫控模組,達到可加工溫度的目的。
而本研究最後成功使用無毒苯二甲藍素顏料搭配120kHz高頻率超音波振盪與超分散劑SOLSPERSETM17000的應用,採用物理加化學分散的方式將顏料粒子大小降低至10μm,染色混合於材料體系內,並以光穿透能量量測,調配最佳染色濃度至2%進行光固化多層疊層,最後以顯微鏡進行支架尺寸量測確認其精度誤差相較於未染色的20%誤差,精度準確控制於5%以內,並以MTT驗證其應用於生物體內並無明顯毒性。
Tissue engineering scaffolds of PCL-DA and PEG-DA will have the over-curing problem when encountering light transmission, because their transparent materials have the feature of optical transmittance. Also, the usage of acetone leads to shrinking problems of photocuring scaffolds. The previous studies have shown that method of scaffold dyeing can improve the problem of light transmission. Therefore, this paper tries to use non-toxic dyes and pigments to replace the original ink dye for the application to fabricate tissue engineering scaffolds, as well as to determine whether the biocompatibility is good based on the cell attached condition for the applied value of tissue engineering. Meanwhile, PCL-DA is melted by heating to melting point in order to mix with PEG-DA instead of adding acetone to solve the shrinking problem caused by acetone in light curing materials. Additionally, due to demands for heating and melting, we set up a material tank and the temperature controlling module in the original DLP dynamic photomask system to achieve the purpose of processable temperature.
In this study, we using the non-toxic phthalocyanine blue pigment preparation by high-frequency ultrasonic oscillations of 120 kHz with hyperdispersants of SOLSPERSETM17000. We utilize physical with chemical dispersing methods to grind the pigment particle size to 10μm. And dyeing is mixed in the material system and measured by the energy of light transmission to choose the best dyeing concentration of 2% to photocuring multilayered scaffolds. In the end, a microscope is used to measure the dimension of scaffold to make sure its accuracy error compared with that on the unstained of 20% as well as the precision accuracy controlled within 5%, and MTT is used to validate that there is no obvious toxicity of its application in an organism.
【1】http://textile.iitd.ac.in/highlights/fol8/01.htm
【2】C. Becker and G. Jakse, "Stem Cells for Regeneration of Urological Structures, " european urolo g y , Volume 51 (2 0 0 7),1217 – 1228.
【3】宋信文 梁晃千 ,“建立人類的身體工房-組織工程” ,科學發展第362期,2003年2月,6-11
【4】H. Y. Kweon, M. K. Yoo, I. K. Park, T. H. Kim, H. C. Lee, H. S. Lee, J. S. Oh, T. Akaike, and C. S. Cho, “A novel degradable poly caprolactone networks for tissue engineering,” Biomaterials,Volume 24 (2003) 801-808.
【5】Andreas Gebhardt,“Understanding Additive Manufacturing,” Hanser Publications ,P.4
【6】H. Jiankang, L. Dichen, L. Yaxiong, Y. Bo, L. Bingheng, L. Qin “Fabrication and characterization of chitosan/gelatin porous scaffolds with predefined internal microstructures,” Polymer, Volume. 48(2007), 4578-4588.
【7】F. P.W. Melchels, J. Feijen, D. W. Grijpma,“A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography,” Biomaterials, Volume. 30(2009),3801-3809.
【8】J. W. Choi, R. Wicker, S. H. Lee,K. H. Choi, C. S. Ha and I. Chung“Fabrication of 3D biocompatible/biodegradable micro-scaffolds using dynamic mask projection microstereolithography”, Journal of Materials Processing Technology, Volume 209(2009), 5494-5503
【9】Laura Elomaa, Sandra Teixeira, Risto Hakala, Harri Korhonen, Dirk W. Grijpma, Jukka V. Seppälä,“Preparation of poly(e-caprolactone)-based tissue engineering scaffolds by stereolithography”, Journal of Materials Processing Technology, Volume 7(2011), 3850-3856
【10】李孟龍,“動態光罩快速原型系統製造組織工程支架之研發”,國立台灣科技大學機械工程研究所,碩士論文,2005。
【11】陳俊豪,“光固化快速成型技術製作組織工程支架之研究”,國立台灣科技大學機械工程研究所,碩士論文,2006。
【12】許貽玨,“光聚合生物可分解材料應用於RP技術製作組織工程支架性質之研究”,國立台灣科技大學機械工程研究所,碩士論文,2007。
【13】陳茂揚 “光固化快速成型系統製作3D組織工程支架”國立台灣科技大學機械工程研究所,碩士論文,2008。
【14】曾俊元“動態光罩快速成型系統光聚合PCL-PEG-PCL製作3D組織工程支架”,國立台灣科技大學機械工程研究所,碩士論文,2008。
【15】薛智仁“動態光罩快速成型系統製作3D PCL管狀多孔性組織工程支架之研究”,國立台灣科技大學機械工程研究所,碩士論文,2009。
【16】謝浚雄“光聚合PCL材料系統成份探討及其應用於快速成型3D組織工程支架”,國立台灣科技大學機械工程研究所,碩士論文,2011。
【17】孫凱閔“PCL結合PEG-acrylate透過動態光罩成型系統製作3D多孔性組織工程支架” 國立台灣科技大學機械工程研究所,碩士論文,2011。
【18】侯佳延“PCL結合PEG-diacrylate透過反射式動態光罩成型系統製作3D組織工程支架”國立台灣科技大學機械工程研究所,碩士論文,2012。
【19】楊淯凱 “以積層製造技術光顧畫PCL-PEG-diacrylate之材料性質與組織工程支架成型性探討”,國立台灣科技大學機械工程研究所,碩士論文,2013。
【20】D. Sengupta, B. Mondal, K. Mukherjee, “Visible light absorption and photo-sensitizing properties of spinach leaves and beetroot extracted natural dyes,” Spectrochimica Acta,Volume148(2015), 85-92
【21】Yi Liu, Lingmin Shao, Jianmin Gao, Hongwu Guo, Yao Chen,Qingzheng Cheng, Brian K. Via, “Surface photo-discoloration and degradation of dyed wood veneerexposed to different wavelengths of artificial light,” Applied Surface Science, Volume331(2015), 353-361
【22】http://www.ti.com/lsds/ti/analog/dlp/overview.page
【23】http://ssnt-tech.com/forum_det.php?action=view&id=77
【24】https://www.pida.org.tw/optolink/optolink_pdf/87111810.pdf
【25】http://projector.sg/index.php?main_page=product_info&products_ id=900
【26】Christian Decker, “Kinetic Study and New Applications of UV Radiation Curing,” Macromolecular Rapid Communications, Volume23(2002), 1067-1093
【27】http://www.sigmaaldrich.com/catalog/product/aldrich/189421?lan g=en®ion=TW
【28】http://www.sigmaaldrich.com/catalog/product/aldrich/455008?lan g=en®ion=TW
【29】http://www.chitec.com/english/tpo.php?gclid=Cj0KEQiA496zBR Doi5OY3p2xmaUBEiQArLNnK_Ww_JBrbjSiy3vhW9HW3dqo4TSepNq7eVGueM1peGcaAkeN8P8HAQ
【30】http://coatings.specialchem.com/product/a-lubrizol-solsperse-1700 0
【31】http://s-sunbio.en.alibaba.com/product/60245570385-801547825/ Gardenia_blue_powder_for_food_colorant_organic_gardenia_blue_powder.html
【32】http://www.sigmaaldrich.com/catalog/product/sigma/m6900?lang= en®ion=TW
【33】http://www.sigmaaldrich.com/catalog/product/aldrich/546682?lan g=en®ion=TW
【34】https://www.youtube.com/watch?v=G30kw533mPY&index=2&lis t=PLZE87uXnozrR5NeeJNJD8_GC4u9Z1XzdV
【35】http://www.aaultrasoniccleaners.com/ultrasonic.htm
【36】http://watersound.com.tw/product/showproduct.php?id=19