肺臟具重要的氣體交換功能，多數肺損傷患者須透過肺臟移植或氧合器之輔助方能維持生理機能，但移植患者需終身服用免疫抑制藥物，組織工程有機會解決現今之問題。然而，肺組織工程所使用之材料不僅須有生物相容性，亦須具備交換氣體功能，目前文獻研究以細胞培養居多，氣體交換相關者較少，且肺臟組織複雜的血管網路與肺泡為複雜三維結構，一般製程難以生產。因此，本研究擬導入積層製造技術與添加維他命E之生物柔韌材料，以建立模擬肺泡模型，並測試其氣體通透性與模擬肺泡膨脹收縮。
因應肺泡於呼吸時會產生體積上的膨脹收縮，材料必須擁有適當彈性及機械性質，因此本研究選擇CT2、PUA_L、IC27三種不同性質之柔韌材料進行探討，並於材料中添加抑制劑維他命E，除可提高列印時的品質，亦有助於固化後的材料之氣體交換性質。材料性質測試包括拉伸試驗、接觸角量測 、TGA、DSC、FTIR、降解測試、體外細胞培養與薄膜氣體透測試，並挑選最適合的CT2添加1% 維他命E，以DLP型3D列印製作200 μm模擬肺泡，測試其膨脹收縮與氣體交換功能。所得的模擬肺泡模型，總表面積為0.01 m2，模擬人體單次呼吸的頻率時，可提升受測區域溶氧濃度0.016mg/L，與人體單次呼吸時所通透的氧氣量數據相近。本研究之成果驗證了材料與製程於模擬肺泡模型之可行性，未來將可進一步應用於肺組織工程。

The lung has an important gas exchange function. Most patients with lung injury require lung transplantation or oxygenator assistance. However, transplant patients need to take immunosuppressive drugs for life. Tissue engineering(TE) has the opportunity to solve the problem. However, the materials used in lung TE must have biocompatibility and the function of exchanging gas. At present, most of the literature studies are based on cell culture, and there are few related to gas exchange. The vascular network and alveoli are complex 3D structures that are difficult to produce in general processes. Therefore, this study intends to introduce additive manufacturing technology and flexible material added with vitamin E to establish a simulated alveoli model and test its gas permeability, expansion, and contraction.
Due to the volume expansion and contraction of the alveoli during respiration, the material must have appropriate elasticity and mechanical properties. Therefore, this study selected three flexible materials with different properties: CT2, PUA_L, and IC27, and added the inhibitor vitamin E to the material. In addition to improving the quality of printing, it also contributes to the gas exchange properties of the cured material. Material property tests include tensile test, contact angle, TGA, DSC, FTIR, degradation test, in vitro cell culture, and film gas permeation test, and select the most suitable CT2 supplemented with 1% vitamin E, which is produced by DLP type 3D printing 200 μm simulated alveoli to test its expansion, contraction, and gas exchange functions. The obtained simulated alveoli model has a total surface area of 0.01 m2. When simulating the frequency of a single breath of the human body, the dissolved oxygen concentration in the measured area can be increased by 0.016 mg/L, which is similar to the data of the amount of oxygen permeated by a single breath of the human body. The results of this study verified the feasibility of materials and processes in mimic alveoli models and will be further applied to lung tissue engineering in the future.

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