本研究是以碳為工作與輔助電極，銀/氯化銀為參考電極製備成三極式拋棄式微型電極做為過氧化氫感測試片。在工作電極上覆蓋一層含鉑-多層奈米碳管(Pt-CNT)觸媒催化過氧化氫，藉此降低施加電位，並增強電流訊號，於施加電位0.3V時，過氧化氫偵測線性範圍為0.01-1 mM與1-15 mM而偵測靈敏度分別為75.2 μA mM-1 cm-2與120.7 μA mM-1 cm-2，而最低過氧化氫偵測極限為0.01 mM，此感測試片不僅可應用於偵測食品中殘留的過氧化氫含量，若在觸媒層上再覆蓋一層FPO (fructosyl peptide oxidase)酵素，則可偵測由糖化纈草胺酸(fructosyl valine，FV)與FPO酵素反應產生的過氧化氫。此製備之FPO酵素感測試片，於施加電位0.3V下，糖化纈草胺酸偵測線性範圍為0.01-0.5 mM，偵測靈敏度為51.4 μA mM-1 cm-2，而最低FV偵測極限為0.01 mM。由於糖化血紅素(HbA1c)在血液中的濃度，可反應出二至三個月的平均血糖濃度，是很好的糖尿病指標，而糖化纈草胺酸又為糖化血紅素的末端糖化官能基，藉由偵測水解過的HbA1c與FPO反應所產生之過氧化氫濃度，可反推出血液中之HbA1c含量，但紅血球中所存在的過氧化氫酶(catalase)會分解FPO反應所產生之過氧化氫，進而影響HbA1c的偵測，若先以離心超過濾前處理血樣，可有效將過氧化氫酶移除，經此法前處理後之紅血球水解物，可以用所製備之拋棄式FPO微型感測試片偵測出其中HbA1c含量。達到快速偵測、成本較低、操作簡便的HbA1c偵測目的。

A single-use H2O2 electrochemical sensor was fabricated by assembling a PtCNT-carbon modified working electrode, an Ag/AgCl reference electrode, and a carbon counter electrode. Pt-CNT catalyst as coated on the carbon-based working electrode to reduce the working potential and enhance the current signal for the H2O2 detection. The working potential for the amperometric detection was fixed at +0.3V versus the reference electrode. Two linear ranges 0-1 mM and 1-15 mM of H2O2 detection were observed and their sensitivities were 75.2 μA mM-1 cm-2 and 120.7 μA mM-1 cm-2, respectively. This single-use sensor not only can be applied to detect H2O2 residual concentration in food, it also can detect the FV(fructosyl valine) concentration. If FPO(fructosyl peptide oxidase) were coate as catalyst layer FV can be detected with sensitivity of 51.4 μA mM-1 cm-2 with linear range 0.01 to 0.5 mM and detection limit of 0.01 mM. In human body, the level of HbA1c can reflect the average blood sugar level of 2 to 3 months. The HbA1c can be proteolysis to generate FV. Therefore, this FV sensor was employed to determine the concentration of HbA1c in blood. However, the catalase in the blood cell will interfere the detection significantly. In this study, vivaspin 500 ultrafiltration was used to remove the catalase from blood sample. After catalase removal, HbA1c concentration can be accurately measured by this single-use FV sensor.

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