古生嗜鹽菌Halobacterium salinarum細胞膜上含有紫膜 (purple membrane, PM) ，其內含有細菌視紫質 (bacteriorhodopsin, BR) ，為一種光驅動質子泵浦，受光激發後其膜兩側會產生質子梯度差，可進而產生光電流。本研究基於BR光電響應與入射光強度呈正向關係之事實，並以核酸適體 (aptamer) 作為生物辨識分子，分別將針對人類血紅素 (hemoblobin, Hb) 與糖化血紅素 (HbA1c) 的核酸適體以架橋固定化於PM晶片上，以分別定量檢測Hb與HbA1c。研究中探討晶片製程及檢測最適化條件，並且以PM感測晶片之光電流與拉曼光譜分析分別確認這兩種核酸適體之固定化及Hb與HbA1c之檢測。在最適化條件下，15分鐘即可完成檢測，且兩種晶片檢測靈敏度均可低達0.1 μg/mL以下。而在實地樣品檢測時，晶片檢測所得的HbA1c值 (HbA1c %) 和標準測量法相比，平均誤差值為2.53±1.44 %，且對每個樣品晶片檢測之RSD %皆在5 %以下 (n=3) 。此外與市售A1CNow 檢測儀器的量測HbA1c %值相比，本研究晶片結果不僅與標準方法之關係稍高，且具有更寬廣檢測範圍，顯示其未來商業化之可行性。

One of the archaeon Halobacterium salinarum cellular membranes is purple membrane (PM) containing bacteriorhodopsin (BR), a light-driven proton pump which generates a proton gradient and thus photocurrent across the membrane upon illumination. Based on the positive correlation of BR photoelectric response with the incident-light intensity, this study first demonstrates a novel quantitative detection method of hemoglobin (Hb) and hemoglobin A1c (HbA1c) by immobilizing their respective aptamers on PM-coated chips to serve as the recognition element. Chip fabrication and Hb/HbA1c detection were both optimally investigated and confirmed by both Raman spectroscopy and PM-photocurrent measurement. Both Hb and HbA1c were sensitively detected at 0.1 μg/mL in 15 min. For real field sample detection, an averaged accuracy of 2.53±1.44 % was obtained for the HbA1c% value, with RSD < 5 % (n=3) for each sample. In comparison with the commercial HbA1c detection instrument, A1CNow, the current PM-based measurement method not only yielded results with a slightly better correlation with what were obtained by the standard method but also exhibited a wider dynamic range, suggesting its future commercial potentials.

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