生醫玻璃(BG)由於具有高度可調節的生物活性、生物相容性和生物降解特性，因此在骨組織工程和骨植入物、牙齒填充材料和藥物載體等潛在應用中受到了廣泛的關注。本文重點研究了噴霧熱烈解法製備生物活性玻璃的微結構、細胞毒性、降解行為和抗菌性能之間的相關性，以研究生醫玻璃的生物相容性和生物降解性質。另外，進行錳(Mn)摻雜的BG是為了克服BG所缺乏的抗菌活性。
在這項研究中，利用X光繞射圖(XRD)來進行BG的相分析鑑定。使用掃描電子顯微鏡(SEM)觀察這些玻璃狀粉末的表面形貌，然後使用透射電子顯微鏡(TEM)確定其內部結構。使用MTT試驗 (3− [4, 5−dimethylthia− zol−2−yl] −2, 5−diphenyl tetrazolinum bromide) 進行細胞毒性的評估。此外，通過將粉末浸入模擬體液中1天，並通過X光繞射，SEM和FTIR技術對其進行了分析，從而分析了體外生物活性。最後，分別通過浸泡在SBF和菌落計數法中進行降解行為和抗菌活性測試。
在第一個實驗中，我們通過使用各種酸催化劑(鹽酸、乳酸和醋酸)進行噴霧熱解來合成生醫玻璃(BG)粉，以控製表面形貌。檢查了表面形貌對所製備的BG粉末的細胞毒性和生物活性的影響。發現光滑的表面形貌表現出更好的細胞活力。特別是用鹽酸處理的(HBG)表現出光滑的表面形貌，也表現出更好的細胞活力。但是，乳酸處理(LBG)和醋酸處理(ABG)對細胞增殖具有抑製作用。
在第二個實驗中，我們使用各種濃度的聚乙二醇(PEG)來控制BG的顆粒形貌，結構和孔隙率。並鑑定了形貌和降解行為的相關性。我們發現，隨著PEG濃度的增加，觀察到更高數量的多孔和中空顆粒，且粉末顯示出更高的比表面積和更高的生物活性。另外，在PEG處理的BG樣品中產生了更多的多孔和空心結構表現出更好的降解速率。
本文的最後一部分提出了各種濃度的錳摻雜BG的製備方法。抗菌活性的結果表明，摻入5mol％ Mn的BG粉末具有更好的抗菌性能，增強了BG粉末的生物活性，有利於新一代組織的發展。

Bioactive glass (BG) receives considerable attention in the bone tissue engineering and wide potential applications for bone implants, tooth filling materials, and drug carriers due to their highly tunable bioactivity, biocompatibility, and biodegradation properties. Herein, the dissertation highlights the correlation of microstructure, cytotoxicity, degradation behavior and the antibacterial properties for spray-pyrolyzed bioactive glass to investigate the biocompatibility and biodegradation properties of bioactive glass. In addition, manganese (Mn) doped BG was performed in order to over comes the lack of antibacterial activity of BGs.
In this study the phase compositions of the BG powders were characterized by X-ray diffraction. The surface morphologies of these glassy powders were observed using scanning electron microscopy (SEM), and then use transmission electron microscopy (TEM) to determine its inner structure. The cytotoxicity was evaluated using MTT test (3− [4, 5−dimethylthia− zol−2−yl] −2, 5−diphenyl tetrazolinum bromide). Furthermore, the in vitro bioactivity properties was analysed by immersing the powders into simulated body fluid for 1day and characterized by X-ray, SEM, and FTIR techniques. Finally, degradation behaviour and antibacterial activity tests were conducted by soaking in SBF and colony counting method respectively.
In the first experiment we synthesized bioactive glass (BG) powders by spray pyrolysis with various acid catalyst (hydrochloric acid, lactic acid and acetic acid) to manipulate the surface morphologies. The effect of surface morphologies on the cytotoxicity and bioactivity of the prepared BG powders were examined. It was found that the smooth surface morphologies exhibited better in cell viability. Particularly, hydrochloric acid treated (HBG) showed smooth surface morphologies which exhibited better cell viability. However, lactic acid treated (LBG) and acetic acid treated (ABG) show an inhibitory effect on cell proliferation.
In the second experiment, we use various concentration polyethylene glycol (PEG) to manipulate particle morphologies, structures and porosity of BG. Furthermore, the correlation of morphology and degradation behavior were examined. We found that with the increase of PEG concentration, higher populations of porous and hollow particles were observed and the powders exhibited a higher specific surface area and higher bioactivity. In addition, the more porous and hollow structures created in PEG-treated BG specimens exhibited better degradation rate.
The final part of the thesis presents the preparations of various doping concentrations of Mn-doped BG. The results of antibacterial activity suggest that, 5mol% Mn-doped BG powders have better antibacterial property and enhanced the bioactivity of BG powders, which benefit to the new tissues generation.

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