利用同軸電流體動力霧化法（Coaxial Electrohydrodynamic Atomization，CEHDA）製備微米粒子可以獲得相對窄的粒徑分布與包覆效率佳的核殼式結構顆粒，於新型藥物釋放系統的發展是更有潛力。
在這項研究中，通過靜電噴霧法製備PLGA、幾丁聚醣微粒和PLGA /幾丁聚醣核殼式微粒，其中藥物被包封在該一步法中。首先，研究影響合成顆粒形態和結構的幾種工藝參數，如流速、電壓和聚合物濃度之參數最佳化。透過掃描式電子顯微鏡照片證明PLGA和幾丁聚醣微粒的平均粒徑分別為1.423μm和0.511μm，而核殼式顆粒大小坐落於1.098μm。為了確定核殼結構，進行了表面介達界達電位與傅立葉轉換紅外線波峰圖譜的分析。結果表明，EHDA成功製備了PLGA /幾丁聚醣核殼式顆粒，程序參數優化為20kV和內流速0.3ml / hr外流速0.45ml / hr。除此之外，共聚焦顯微鏡下的染色結果與穿透是顯微鏡也證明了雙層的存在。
核殼式顆粒中，核心含有低濃度的Simvastatin，釋放曲線表明使用PLGA /幾丁聚醣核殼式顆粒可以達到長期緩慢釋放，核殼顆粒初始釋放Simvastatin的速率僅為PLGA顆粒釋放速率的0.1倍，表示沒有初始突釋現象的發生。
最後，細胞的結果證實顆粒的生物相容性良好，本研究中開發的PLGA /幾丁聚醣核殼式顆粒將具有潛力成為骨質疏鬆藥物載體。

Electrohydrodynamic atomization (EHDA) is potential improvement over other technologies for the preparation of particles. It is used to produce particles with a narrow size distribution from micro to nanometer sizes, and would be able to cause less damaging effects and better loading efficiency on encapsulated drugs.
In this study, PLGA, chitosan microparticles and PLGA/chitosan core-shell microparticles were prepared by electrostatic atomization, where drugs were encapsulated in this one-step process. First, several process parameters such as flow rate, voltage, and polymer concentration, which would affect the morphologies and structures of synthesized particles, were investigated for optimization. SEM images proved that the uniform particles were obtained with the average diameter of 1.423 μm and 0.511 μm for PLGA and chitosan microparticles. Meanwhile, the diameter of core-shell particles is 1.098μm. To identify core-shell structures, the analysis of zeta potential and FTIR was carried out. The results showed that PLGA/chitosan core-shell particles were successfully fabricated by coaxial EHDA with optimized process parameters as 20kV, inner flow rate of 0.3 ml/hr and outer flow rate of 0.45 ml/hr. The staining results under confocal microscope proved the existence of double layers, too.
In the core-shell particles, the core of them contains a low concentration of Simvastatin, The releasing profile in buffer solution indicated that a long-term releasing would be reached by using PLGA/chitosan core-shell particles.
Finally, the osteoblasts (7F2) were cultured with these nanoparticles and the viability of cells was analyzed. The results supported that the biocompatibility of particles was good. This study demonstrated that the PLGA/chitosan core-shell particles developed in this research would have the potential to become osteoporosis drug carriers.

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