本實驗將以CA膜(cellulose acetate) 經雙氧水(H2O2)氧化處理後，在膜表面成一帶有羧基 COOH 之凝膠層，再以水溶性二亞胺基羰 EDC 活化表面上羧基，然後與銀杏內酯B(Ginkgolide B)接合，期望改質之後的CA膜會有更好的血液及生物相容性，並能提高凝血時間，以作為血液透析材料之應用，藉以改變以抗凝血劑肝素之給予方式，以減少對人體之不相容性。本實驗利用分別APTT, PT, FT, 凝血時間,纖維蛋白吸附與血小板吸附與形態觀察評價材料之血液相容性,以及分析其血液凝固路徑,由實驗結果顯示PT及FT凝血時間有明顯的提升由血小板的吸附與形態觀察分析亦可證明接枝銀杏內酯B之薄膜不使血小板活化以及吸附在材料的表面。由實驗結果證明以銀杏內酯B接枝於薄膜的表面顯示出良好的抗凝血效果。

In this study, cellulose acetate (CA) membrane were oxidized with hydrogen peroxide to introduce carboxyl groups, which in turn were immobilized with Ginkgolide B(GB). Blood compatibility was evaluated by measuring the activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen time(FT), as well as the activation and adhesion of platelet. The protein affinity was determined by human plasma fibrinogen(HPF) The results showed that the immobilizing amount of GB increased with the density of carboxyl groups, and After grafting Ginkgolide B,the PT and FT was greatly elongated. These results indicated that the blood compatibility of CA membrane could be improved by the immobilization of Ginkgolide B.

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