本研究開發一可整合於液相層析串聯質譜儀系統之3D列印微流道晶片，用來檢測濫用毒品。傳統上在利用液相層析串聯式質譜儀(LC-MS/MS)系統分析待測物時，需使用層析管柱來分離待測物中的不同成分來分辨待測物中的化學物質；透過相關文獻探討後發現越來越多研究團隊投入微流體製程並將其整合於相關物質檢測系統上，開發相關物質檢測技術應用於臨床醫療以及檢測工作，然而有鑑於傳統微流體製程所製作的裝置無法耐受層析過程中所衍生的高壓，因而容易發生洩漏露或是結構破壞的狀況，因此，本研究欲利用微製程技術開發一可耐高壓達1500-2000psi之微型化層析微流晶片，以克服上述應用上的問題，藉由聚合物材質的耐壓性與堅韌性，製作一可連續上機使用3,024分鐘的微流道晶片，且僅需花費較低的成本以解決目前低流速液相層析質譜儀偵測濫用毒品之瓶頸。
針對層析微流晶片製程，本研究利用光固化3D列印技術製作微流晶片本體，搭配自行開發之兩種層析固定相粒子(PFPP,HILIC)填充與封裝技術，製作檢測更多樣性毒品的層析微流到晶片，並藉由14種毒品檢體上機進行晶片穩定性測試，證明層析晶片上機過程中壓力維持穩定且訊號滯留時間維持毒理標準(AAFS-SOFT 2006)之2%內，證明層析微流道晶片不僅於高壓層析環境有高度程度穩定性且成功地將毒品檢體進行層析分離。

A microfluidic chip coupled to a liquid chromatography-mass spectrometry (LC-MS) system is used to determine components of interest in drug samples. However, producing a reusable and robust chip for LC-MS systems remains analytically challenging due to the high back pressure. In this study, a one-piece and high-pressure-resistant polymeric microfluidic chip was additive manufactured and coupled to regular LC-MS. Experiments results demonstrated the following conclusions: (1) the customized additive manufactured polymeric microfluidic device can be connected to a regular LC-MS at low flowrate, forming an integrated system, and operating under a high pressure between 1500~2000 psi. (2) Applying constant high pressure, continuously pressure change or high organic composition to the system, the chip remained intact based on scanning electron microscopy findings and can be used for over fifty hours (approximately three-hundred runs) without leakage. (3) The platform performed proper stability and can be reused to analyze 14 abused drugs without contamination, which fits for clinical/forensic toxicology purpose in 2%. The experimental results listed above prove that the customized additive-manufactured polymeric microfluidic chip integrated with LC-MS can be an efficient and precise tool for bioanalysis.

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