本研究先以縮水甘油三甲基氯化銨(glycidyl trimethyl ammonium chloride,GTMAC)及N-(2-羥基)丙基-3-甲基二丙烯基四級銨鹽 glycidyl-methyl-diallyl amonium salt (GMDAS)修飾幾丁聚醣，合成N-(2-羥基)丙基-3-三甲基氯化銨幾丁聚醣(N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride) (HTCC)及N-(2-羥基) 丙基-3-甲基二丙烯基四級銨鹽化幾丁聚醣(HMDC)水溶性幾丁聚醣衍生物。再將沒食子酸(gallic acid)與HTCC及HMDC進行接枝反應，合成所得之HTCC-GA及HMDC-GA水溶性幾丁聚醣衍生物。
使用FT-IR分析幾丁聚醣修飾物HTCC、HMDC、HTCC-GA及HMDC-GA，另以接觸角、膨潤度及zeta potential分析幾丁聚醣及其修飾物之物性，此外，並以紫外光/可見光分光光譜儀測定HTCC-GA及HMDC-GA之總分含量及DPPH自由基清除能力，最後進行抗菌性與細胞相容性實驗，並期望可應用至傷口敷料上。

Chitosan was modified with glycidyl trimethyl ammonium chloride (GTMAC) and glycidy methyl diallyl ammonium salt (GMDAS) to produce two water soluble derivatives, N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) and N-(2-hydroxyl) propyl-3-methyl diallyl quaternary ammonium salts of chitosan (HMDC). Furthermore, HTCC and HMDC were modified with gallic acid to produce two water soluble derivatives, HTCC-GA and HMDC-GA.
In the first part of this study, HTCC, HTCC-GA, HMDC and HMDC-GA were examined using FT-IR to determine the chemical structures. The hydrophilicity was evaluated based on the water contact angle, swelling ratio, and biodegradability. The surface charge was determined using the zeta potential. The antibacterial activity, and cytocompatibility were evaluated for the applications as wound covering material.

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