目前治療雄性禿（androgenetic alopecia）常見的方法包括口服柔沛（finasteride）、外用生髮水（minoxidil）與植髮（hair transplantation），但這些方法的效果因人而異。近年學者研究發現，將混合的真皮乳突細胞與表皮細胞植入禿髮處，可促進毛囊再生。但現階段的細胞植入方式多採用手術或注射器等方式植入細胞，手術過程耗費大量時間在製備及裝填細胞的步驟。為了縮短前置作業時間、加快植入速度與固定植入深度，本研究開發具有兩種細胞儲存能力的連續式細胞植入器。
連續式雙管細胞植入器的設計概念是以注射筒做為儲存細胞的容器，且植入器可依照預期的順序植入細胞。兩種細胞分別裝填於注射筒內，將完成細胞裝填之注射筒安裝於細胞植入器上，藉由直線步進馬達及對應的機構使注射筒透過針具進行細胞的植入，並採用連桿滑塊機構與馬達達到交替兩個注射筒的效果。連續式雙管細胞植入器之各零組件於3D繪圖軟體中繪製完成後，即以積層製造技術中的SDM (Shape Deposition Manufacturing)製程與PolyJet™技術製作出原型零件。將組合完成之原型於裸鼠身上進行模擬植入測試。利用紅色與藍色兩種顏色之染劑分別做為兩種細胞之模擬，將其植入裸鼠背部，觀察表面情形，隨後取下其植入區域之皮膚，觀察植入效果並進行評估。測試結果發現連續式雙管細胞植入器能夠成功達成兩種細胞於同一位置之植入，並可依照需求控制兩種細胞的植入順序與植入量，針具與注射筒替換容易、迅速，且其具有良好的植入效果與植入成功率。

The general treatments of androgenetic alopecia include finasteride, minoxidil, and hair transplantation. But the effects are still patient-dependent. In recent years, scholars have found that implanting mixtures of dermal papilla cells and epidermal cells into bald areas could promote hair follicle regeneration. However, most cell implantations nowadays use surgeries or manual syringe-ejection, which are time-consuming in preparations and cells filling. To shorten the lead time, to accelerate the implantation speed, and to control the implantation depths, this study planned to develop a continuous double-syringe cell implanter that can store and implant two kinds of cells.
The design concepts of this cell implanter utilize syringes as containers for cells storage, and the implanter can implant cells in the expected orders. In particular, two kinds of cells were filled in the syringes separately and loaded in the implanter. Through the linear actuators and corresponding mechanisms, the syringe was engaged to the needles and ejected the cells. The slider-crank mechanism and the motor were adopted to alternate the two syringes. The components of the implanter was designed in the 3D CAD software and fabricated by SDM (Shape Deposition Manufacturing) process or PolyJet™ technology, ones of the Additive Manufacturing techniques. The assembled prototype was used to perform simulated implantation tests on nude mice. Red and blue gels, imitating two kinds of cells, were implanted into mice’s back. Observations were conducted on the back’s surfaces and also from the inside surface after cutting off the skin. The results showed that the continuous double-syringe cell implanter can successfully implant two kinds of cells into the same areas. The implantation orders and quantities are controllable. Moreover, the steps of exchanging needles and syringes are easy and fast, and the implantation effects and successful rates are satisfying.

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