研究生: |
陳冠宇 Guan-yu Chen |
---|---|
論文名稱: |
非酵素電流式尿酸生物感測器之製備 Fabrication of a Non-enzymatic Amperometric Uric Acid Biosensors |
指導教授: |
王孟菊
Meng-jiy Wang |
口試委員: |
林俊成
Jing-cheng Lin 王文 Wen Wang |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2012 |
畢業學年度: | 100 |
語文別: | 中文 |
論文頁數: | 107 |
中文關鍵詞: | 非酵素 、尿酸 、生物感測器 |
外文關鍵詞: | non-enzymatic, uric acid, biosensor |
相關次數: | 點閱:284 下載:1 |
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尿酸 (uric acid) 是人體內嘌呤代謝 (purine) 的最終產物,物質經由肝臟代謝後存在於血液及尿液中,若濃度過高易引發痛風 (gout)、高尿酸血症 (hyperuricemia) 等疾病。高尿酸血症也與許多疾病相關,例如:慢性腎病、代謝症候群、高血壓以及心臟病。因此監測血液或尿液中尿酸的濃度是掌握病況的重要方法。由於傳統尿酸分析方法繁複,所需時間也較長,且需要專業人員判讀。故近期朝著簡易輕便快速的方式檢測。因此本論文使用電化學中的電流式方式製備尿酸感測器,由於分析儀器的小巧靈敏,操作方式簡易,使用電化學方式測尿酸之研發也日新月異,逐漸成為市面上主要針測尿酸的方式。
本論文使用網印製程,配合濺鍍技術,在聚對苯二甲酸乙二酯 (polyethylene terephthalate, PET) 基材鍍上一層的金屬薄膜,再利用舉離製程,成功製備出二極式的金屬電極。接著各別濺鍍兩種金屬薄膜,鈦、鎳鉻作為導電材料。相較於傳統使用的貴金屬材料,例如:金、白金等,製備花費相對的低。在偵測尿酸方面是使用非酵素方式,利用電子傳遞物質 (ferricyanide) 會直接與尿酸產生電子轉移現象,接著電子傳送至金屬電極表面,達到訊號的偵測。除了比起傳統使用尿酸酶偵測尿酸,在製備上簡易且穩定外,使用電子傳遞物質也可有效的降低偵測電位,使得血液中的電活性物質的干擾訊號降低。例如:維他命C (ascorbic acid, AA)、對乙酰氨基酚 (acetaminophen, AAP) 。
本論文中利用循環伏安法以及計時安培法偵測尿酸,最佳化感測配方使用於金/鈦、金/鎳鉻金屬電極,得到靈敏度分別為60, 56 μA/(mM•cm2);R-square分別為0.996、0.999;感測線性範圍分別為4-30, 4-40 mg/dL;變異係數分別為5.44, 4.52 %。實際運用人體血液的偵測,呈現高選擇性,且干擾物質訊號並不影響尿酸的偵測,室溫下保存時間可達至21天。
Uric acid (UA) is the primary end product of purine metabolism, and metabolized by liver present in blood and urine. High levels of UA in the blood (hyperuricemia) are linked with gout and other disease including renal disease, metabolic syndrome, high blood pressure and heart disease. The ability to monitor blood UA level would be helpful in the follow-up of hyperuricemic patients to give them adequate feedback and treatment guidelines. Due to the traditional method for dectecing UA is complicate and requires professional interpretation, the finding of portable UA sensors is important. Therefore, recently more methods were focused to develop using electrochemical method which provide advantages such as simple measurement, short response time, and high sensitivity.
In this thesis, a two-electrode strip was fabricated using thin films technology, followed by lift-off and screen-printing to pattern the electrodes. The titanium (Ti) and nickel-chromium (Ni-Cr) metal layers were sputtered on PET substrate. The electrodes cost less than those electrodes made by precious metals such as gold and platinum. The proposed method for detecting UA is direct electron transfer by ferricyanide. Therefore the prepared electrodes showed advantages of simple fabrication, high stability and reduction of the applied potential.
In this thesis, the UA was dectected using cyclic voltammetric and amperometric methods. The optimal sensing ink was applied in the two metal electrodes, Ti and Ni-Cr, respectively. The range of detected UA showed a linear relation between 4 to 30 and 4 to 40 mg/dL, which possess regression coefficient of R = 0.996, 0.999, respectively. The sensitivity of the UA biosensor was 60, 56 μA/(mM•cm2), and the average coefficient of variation was 5.44, 4.52 %, respectively on Ti and Ni-Cr coated electrodes. Furthermore, the detection of UA in human body has good selectivity, and the storage time can reach 21 days.
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