在傳統的支架製造方法上，普遍有著孔洞大小、分布不易控制、特定外形難以製作，以及冗長的製作時間等問題，皆造成支架製作使用上的不便利；然而以快速原型(Rapid Prototyping)技術應用在製作支架上，以層加工堆疊成型，可有效地解決傳統製作支架的問題。
本研究將延續實驗室先前所研發的生醫動態光罩快速成型系統(Dynamic Mask Rapid Prototyping System)，嘗試利用DLP(Digital Light Processing)投影機內部120W超高壓水銀燈泡，取代外接式光源-光纖點光源機，當作光聚合生醫材料固化的光源，在經濟上的考量也更具效益。以此新光源系統製作支架最佳的孔洞尺寸約為500μm，雖比前光源系統製作孔洞尺寸來得大，但此新光源系統所製作出之支架，經酒精沖洗後處理，孔洞其3D效果尤佳且具孔洞連通性。
針對所使用的生醫高分子材料，PEG-HEMA/PLGA 200型與PEG-HEMA/PLGA 600型，進行材料降解測試，以作為將來應用的評估。200型與600型生醫材料其含水率在第18天有開始增加的趨勢；pH值方面，200型生醫材料在第24天開始，其酸鹼值降至pH7以下，直至第42天才又回升到pH7以上，而600型始終維持在pH7以上；重量損失率方面，記錄60天的測量結果看來，200型的重量損失比600型來得快。
以MG63類骨母細胞(human osteoblast-like cell)、骨髓細胞(bone marrow)進行體外培養，經由倒立式顯微鏡、電子顯微鏡、細胞計數以及生物毒性反應測試等檢測細胞生長於支架上的形態，雖不見細胞在支架上的生長，但由生物毒性反應測試及材料性質檢測可以判斷細胞在培養期間還是有生長的跡象。

Traditional methods to generate tissue engineering scaffold have encountered issues such as limited control of pore-size, restricted geometric shapes, and long fabrication periods. Layered manufacturing techniques, also known as Rapid Prototyping (RP) processes, provide a great opportunity for fabricating scaffolds without above problems.
The research continued the project of Dynamic Mask Rapid Prototyping System developed in our laboratory to cure biodegradable material to generate scaffolds. Instead of using an expensive fiber-type spot UV light source, the internal 120W bulb of the Digital Light Processing (DLP) projector was utilized as a light source. With this approach, no addition light source was required and the cost of the system could be reduced. The best pore size of scaffolds achieved by this new system was about 500μm. Although the pore size was larger than the previous work, the 3D interconnected pore network could be achieved after flushing the fabricated scaffolds by alcohol.
Degradation tests of the fabricated scaffolds were conducted in order to compare the two scaffold materials, PEG-HEMA/PLGA type 200 and type 600, used in this research. The water absorption ratio for both types started to increase after 18 days. The pH value of type 200 dropped below 7 starting from the 24th days and bounced back to above 7 after 42 days, while for type 600 it maintained above 7. The mass lose ratio of the type 200 is faster than the type 600 during 60 days.
MG63 human osteoblast-like cells and bone marrow were cultured on porous scaffolds in vitro for three weeks. The cell growth morphology was observed by optical microscope and SEM, and evaluated by MTT assay kits. Although the cell growth was not able to be observed on the scaffolds after three week, the results of MTT assay kits and nature measuring in 3-day period showed that the cell did growth during culture.

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