Limnophila aromatica 在東南亞常被用來當作香料以及藥草，在文獻記載中，Limnophila aromatic被認為含有豐富的抗氧化物質。因此如何從此植物中獲得抗氧化物質是此類型研究的最終目標。本研究利用索式萃取法以非極性的正己烷和低極性的乙醚當作溶劑，將色素，油酯以及雜質(不具有抗氧化性物質)去除，再利用乙酸乙酯，乙醇和甲醇萃取抗氧化性物質，並和只利用乙醇當作溶劑的浸漬法結果做比較。從抗氧化性物質含量的分析以及DPPH自由基測試的結果中可以發現，在經過色素及油脂的去除後，乙酸乙酯以及乙醇的萃取物中每單位Limnophila aromatica 中所含的抗氧化性物質比例明顯上升。另外從HPLC-DAD的圖譜中也觀察到索式萃取法和浸制法比較下，更可以有效的將非極性的部分去除，藉以獲得更有活性的物質

Limnophila aromatcia is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, Limnophila aromatic was extracted by serial exhaustive Soxhlet extraction methods. Hexane, diethyl ether, ethyl acetate, ethanol and methanol (non-polar to polar) were used as the solvents in the serial exhaustive extraction method. Maceration by aqueous ethanol was carried out as the comparison. The total antioxidant activity, total flavonoid content, total phenolic content and HPLC-DAD analyses of the extracts from the maceration using 75% aqueous ethanol and serial exhaustive method were investigated in this study. From these results, the serial exhaustive extraction method demonstrated more active antioxidant compounds by removing or decreasing the content of less active non-polar compounds in L. aromatica.

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