本研究將開發一款可用於偵測卵巢癌抗原之超敏感度電化學雙陣列感測器，此感測器是由還原氧化石墨烯(reduce Graphene Oxide, rGO)為主體所構成。藉由還原氧化石墨烯的良導電特性以電漿技術進行表面改質，並利用甲苯胺藍(Toluidine blue ,TB)與普魯士藍(Prussian blue ,PB)的合成，增加rGO表面之官能基種類與數量，進一步與卵巢上皮癌細胞所產生之特定蛋白質抗原(CA125與CK19)進行化學鍵之結合，並以微分脈衝伏安法(DPV)同時間地進行電化學測試並交叉分析建立其關係式。
初步研究顯示，經由特定介質的電化學還原反應，其反應機制分析之後可偵測極微量約< 10-14 g/ml的濃度變化，相對於傳統免疫定量分析方法能低於10-6 g/ml(約1 U/ml)濃度，其靈敏度差異極大。此偵測方法之開發可瞭解當卵巢癌患者之血清CA125遠低於正常值時(如< 35 U/ml )的追蹤情形，並對於偵測低濃度CA 125建立其意義性，且在本研究中也發現在不同型態下之細胞所產生之CA125抗體也有著不同性質，其濃度的變化、型態病情復發及嚴重程度等有著密切相關。
本研究將會提供一個極新極靈敏的模式裝置，應用在卵巢癌細胞線ES-2、HeyA8及HeLa之細胞萃取液與患者血液中萃取之血清檢測，並建立其抗原與CA125與CK-19在卵巢癌患者與健康者之表現性，將來並進一步針對此裝置發展成可上市之產品應用於臨床研究上。

This study developed an ultra-sensitive electrochemical multiple bio-sensor device. It can be used to detect ovarian cancer. This sensor is composed of reduced graphene oxide (rGO) by a substrate. The conductivity of graphene oxide can be increased by modifying the surface of rGO using electric-plasma technology. As well to increase the types of functional groups on the surface of rGO by synthesizing the Toluidine Blue (TB) and Prussian Blue (PB). Furthermore, the specific protein antigens (CA125 and CK19), that produced by ovarian epithelial cancer cells, is combined on the rGO surface.
Using the differential pulse voltammetry (DPV) electrochemical tests simultaneously and cross-analysis to establish its relationship.
Preliminary studies have shown that the electrochemical reduction reaction of a specific medium can detect a very small concentration change about <10-14 g/ml. While traditional immuno quantitative analysis methods only can detect about 10-6 g/ml. It shows the significant difference of sensitivity between these methods.
The developed detection method has significant improvement to detect low concentration of CA125, because it can be used to track the patient conditions even the serum CA125 of ovarian cancer patients is below the normal value, such as <35 IU/ml.
Other than that, in this study also found that the CA125 antibodies produced by different types of cells will have different properties, such as concentration and the type of recurrence and severity of disease.
This study provides a novel sensitive model device, which is used in the detection on ovarian cancer cell lines, such as ES-2, HeyA8 and HeLa cells and serum extracted from patients' blood. Establishes its antigen and CA125 and CK-19 in the performance of ovarian cancer patients and healthy people. In the future, it will be developed into marketable products for clinical research.

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