本研究使用非類固醇抗發炎藥物酮基布洛芬 (ketoprofen) 作為測試藥物，以羧甲基纖維素 (carboxymethyl cellulose, CMC) 作為乘載藥物的薄膜，並藉由混摻不同比例的氧化石墨烯 (graphene oxide, GO) 於聚乙烯醇 (poly(vinyl alcohol), PVA) 薄膜中，增加複合材料結構上的立體障礙，以控制藥物釋放的速率，而所製備的複合材料具有良好的吸水能力，可以維持傷口濕潤的環境，促進細胞的遷移及增生，本論文以體外的細胞測試評估作為傷口敷料之可行性後，選出適當的組別進行傷口敷料之活體動物實驗，觀察其應用於開放性傷口上之特性。
　　以掃描式電子顯微鏡 (scanning electron microscopy, SEM) 觀察複合材料的截面結構，結果顯示截面出現不規則的皺摺，可得知氧化石墨烯均勻分散於聚乙烯醇結構中。以Franz diffusion cell進行藥物釋放試驗，為期24小時的藥物釋放週期，結果顯示，未使用薄膜進行不同藥物濃度的釋放試驗，t50 (time taken to release 50 % of the drug) 的平均值約為0.9小時，而混摻1、3及5 wt.%的氧化石墨烯於聚乙烯醇中，t50分別為2.8、6.7及14.9小時，顯示藥物釋放的速率可藉由複合薄膜成分進行調控。在細胞培養測試方面，經由MTT assay分析結果得知，不同混摻條件的薄膜對於老鼠纖維母細胞 (L-929 fibroblasts)，均無明顯的細胞毒性，而LDH assay結果顯示，氧化石墨烯含量增加使得細胞容易貼附及增生於材料表面，證實複合材料具有良好的生物相容性。
　　以上實驗結果顯示，本研究製備之複合材料，除了能減緩藥物釋放的速率，還具有良好的生物相容性， 並且能維持傷口環境的濕潤，具有應用於傷口敷料的潛力。

In this work, the drug release system is adopted to incorporate ketoprofen in carboxymethyl cellulose (CMC) matrix to prepare thin film for drug delivery. The aim of this study is to control the drug diffusion rate by controlling the amount of graphene oxide (GO) in poly(vinyl alcohol) (PVA) to form PVA/GO composites. To assay the biocompatibility of PVA/GO composites, cellular behavior on the membranes was analyzed using LDH assay and MTT assay to observe the results of cell proliferation and adhesion. Finally, in vivo animal experiments using PVA/GO composite were performed on mice to investigate the effects on the wound healing.
Scanning electron microscope (SEM) graphs showed that the cross-sections of the composites present an irregular surface after blending with GO. In vitro release experiments using composites showed that the increase of the concentration of GO in PVA can delay the drug diffusion rate. When seeding the cells (L-929 fibroblasts) on the PVA/GO composites, in vitro LDH and MTT assay results indicated that the composites have excellent biocompatibility.
These results showed that the PVA/GO composites can reduce the drug release rate and showed good biocompatibility. The composites maintained a moist environment with the potential application to the wound dressing.

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