本篇研究的目標是以RGD胜肽和β-D聚醣修改人工生物可分解之PLLA薄膜的表面,藉此提升人類似成骨細胞(MG-63) in vitro的生長。RGD胜肽藉由電漿處理再以EDC/NHS活化而連接到表面。修飾過的PLLA薄膜之表面性質評估包括水接觸角、FTIR、SEM、AFM、coomassie染色法和MG-63細胞附著性質。
此外, MTT分析法顯示電漿處理過的PLLA有顯著的改變，而RGD胜肽對細胞增生的影響較小。總而言之，本研究之結果顯示電漿處理、膜的表面形貌、RGD覆層均為影響細胞附著以及增生之重要因素。

The aim of this study was to modify the surface of synthetic biodegradable PLLA films with RGD peptide and β-D-glucan in order to promote growth of human osteoblast-like cells (MG-63) in vitro. RGD peptide was coupled onto the surface via plasma treatment combined with EDC/NHS activation. The surface properties of various modified PLLA films and membranes were examined included water contact angle, FTIR, SEM, AFM, coomassie staining method, and MG-63 cell adhesion property was evaluated using MTT assay. Furthermore, the MTT assay showed a significant result on PLLA with plasma treatment and also with membrane morphology, while RGD peptide influences cells proliferation to some extent. In summary, the results of this study indicated that plasma treatment, topography of the membrane and the coating of RGD were important factors affecting the cell adhesion and proliferation.

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