聚(氮-異丙基丙烯醯胺)〔Ploy(N-isopropylacrylamide)，PNIPAAm〕具有溫度敏感性，能隨環境溫度變化而進行相對應的變化，為了製備具有溫敏性與生物相容性的複合材料，本研究透過氫鍵作用力將聚(氮-異丙基丙烯醯胺)/去氧核醣核酸(PNIPAAm/DNA)自組裝形成生物複合材料，並探討PNIPAAm與DNA不同混摻比例對生物複合材料氫鍵作用力與結合常數的影響。
應用TGA與DSC對複合粉末進行熱分析，所有比例的PNIPAAm/DNA只有單一的玻璃轉移溫度且互溶成為一均勻相；使用UV測試PNIPAAm/DNA的氫鍵作用力，DNA含量越多則氫鍵作用力越大；使用FE-SEM、FEI-TEM觀測其結合型態與結構，結果發現DNA含量越多顆粒越容易產生聚集現象；經由CA量測發現在25℃時PNIPAAm/DNA隨DNA比例增加而顯現越疏水之特性，在45℃時則會隨DNA比例增加而顯現其越親水之特性；利用DLS探討溫度變化對PNIPAAm/DNA粒徑的影響，其粒徑會隨溫度升高而下降；以SDA進行電傳導性質的分析，結果發現PNIPAAm可增加DNA的導電率。
最後使用點擊反應(Click Reaction)將PNIPAAm接枝於矽晶片表面，並探討PNIPAAm高分子刷在不同溫度下抓取DNA的能力，XPS結果指出，PNIPAAm在LCST以下抓取DNA的量多過於在LCST以上抓取的。透過測試生物複合材料的氫鍵作用力與結構型態變化，期望能成為一種應用於基因轉染與生物晶片的新型複合材料。

Hydrogen bonding between complementary bases of nucleic acids plays a key role in self-aggregation processes and the spatial arrangement of biological macromolecules. This unique supramolecular performance of nucleic acids has stimulated us to explore the possibilities of utilizing these features for the development of advanced artificial systems for various applications.
The hydrogen-bonding interactions and the biocomposites poly(N-isopropylacrylamide) (PNIPAAm) blended with herring sperm DNA were studied on the function of the blend composition by using thermogravimetry analyzer (TGA), differential scanning calorimetry (DSC), ultraviolet−visible (UV-Vis), scanning electron microscope (SEM), transmission electron microscope (TEM), contact angle (CA), dynamic light scattering (DLS) and semiconductor device analyzer (SDA).
PNIPAAm/DNA exhibit a sharp but continuous transition at around 32 °C, from an elongated coil to a collapsed compact state. The PNIPAAm brushes were grafted on the silicon surface by using click reaction. According to the thermo-responsive property of PNIPAAm brushes, the capture-release application for herring sperm DNA was investigated by X-ray photoelectron spectroscopy (XPS).

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