本研究是藉由原子轉移自由基聚合法( atom transfer radical polymerization，ATRP)，將聚甲基丙烯酸N,N-二甲氨乙酯 (Poly (2-Dimethylaminoethyl Methacrylate)， PDMAEMA)高分子刷接枝於矽晶片表面。
使用化學分析之X射線光電子能譜儀(X-ray photoelectron spectroscopy，XPS)，證實表面自組裝起始劑及PDMAEMA高分子成功接枝於矽晶片表面；使用高解析熱電子型場發掃描式電子顯微鏡(high-resolution thermal field emission scanning electron microscopy，HRFE-SEM)與原子力顯微鏡(atomic force microscopy，AFM) 觀看PDMAEMA高分子刷表面形貌與高分子刷粒徑，經不同聚合時間2、4、6、8小時的高分子刷，其粒徑從10nm至25nm。並用表面輪廓儀(nano measurement instrument for surface roughness，Alpha-Step)測量PDMAEMA高分子刷之厚度，其厚度從90.6nm至329.3nm。由接觸角的量測證實在pH=1時PDMAEMA高分子刷呈現延展型態，而在pH=11時PDMAEMA高分子刷呈現捲曲型態。另外在反覆的酸鹼度溶液測試下，PDMAEMA高分子刷具有酸鹼應變可逆行為。
利用PDMAEMA高分子刷之末端基帶有正電荷( R4N+)基團，將奈米金離子吸附於分子鏈上，再還原成奈米金粒子。根據證實甲苯的合成方式比去離子水的合成方式吸附奈米金粒子更多，此外，將合成於甲苯的奈米金粒子晶片浸泡於不同pH值，奈米金粒子呈現的型態也有所不同，並具有奈米金粒子型態回復的特性。藉由固體表面界面電位分析儀(Delsa nano solid interface surface zeta potential measurement system)測量其PDMAEMA與奈米金粒子表面電位，其電位-10.36至-19.98 mV。另外，測試PDMAEMA高分子刷的能力，將鯡魚精子DNA(Herring sperm DNA) 10μg/μl 分子以物理吸附方式附著於高分子刷上。經實驗證實，PDMAEMA高分子刷在pH=7溶液中會抓取DNA分子，而pH=3溶液則會釋放出DNA分子。由結果得知， PDMAEMA高分子刷可作為一種新的特定DNA的萃取方式。
運用微影技術在矽晶圓製備出線寬比為1：2(400nm：800nm)的圖案化的光阻層，利用圖型化光阻之矽晶圓進行起始劑自組裝後，並用丙酮清洗光阻層，即可合成圖案化高分子刷。藉由圖案化高分子刷可進一步應用在生物微機電和半導體製程上。

Poly (2-Dimethylaminoethyl Methacrylate) (PDMAEMA) polymer brushes were grafted on the silicon surface by using atom transfer radical polymerization (ATRP). The silicon surface was treated by oxygen plasma, causing the sueface to become chemically modified (hydroxyl groups). 3-aminopropyltriethoxy silane (APTES) was used to react with the hydroxyl group on the surface, α-Bromoisobutyl bromide reacted with NH2 group of APTES to form halogen groups on chain ends to be the initiator of atom transfer radical reaction. Sequentially, PDMAEMA brushes were grafted on the silicon surface.
Electron Spectroscopy for X-ray photoelectron spectroscopy (XPS) was utilized to verity the surface element of initiator and PDMAEMA polymer brushes for each stage. We observed coil-like structure with 10nm, 13nm, 19nm and 25nm of diameters for PDMAEMA brushes after grafting for 2, 4, 6 and 8 h by atomic force microscopy (AFM) and high-resolution thermal field emission scanning electron microscopy (HRFE-SEM). Approximately linear increase in thickness of the grafted PDMAEMA layer from 90.6 to 329.3 nm was observed on the surface upon increasing the polymerization time to 8 h. Furthermore, the patterned PDMAEMA brushes with width of 400 nm were fabricated by using lithography on the silicon wafer. The patterns of PDMAEMA brushes matched perfectly with the patterns from lithography, indicating that the patterned PDMAEMA could be fabricated by using a very-large-scale integration (VLSI) system to approach commercial application.
Contact angles on the PDMAEMA layer increased to 61.5°at PH=1, but decreased to 72.5°at PH=11, indicating a PH responsive property on the surface. At a PH of 1, the tethered PDMAEMA stretched its polymer chains to increase the hydrogen bonding between water and polymer to create a hydrophilic surface. Upon increasing PH to 11, the tethered PDMAEMA collapsed due to disappearance of positive charges, increasing hydrogen bonding among polymer to create a hydrophobic surface. The PH responsive behavior of PDMAEMA polymer brushes repeated three cycles to verity the property. Moreover, PDMAEMA layer was used to be a extractor on the surface through the PH responsive property. The positive charge on the PDMAEMA attracted a herring sperm DNA with 10μg/μl at PH = 7, a DNA capture process. Upon increasing PH to 3, the positive charge groups disappeared, causing the release of the DNA. The results suggests that The results suggest that tethered PDMAEMA brushes could be a extractor to miniaturize cartridges of sample preparation for rapid disease diagnosis through fluid device to approach the lab-on-a-chip (LOC).
The positive charge (R4N+) groups on the side chain of PDMAEMA brush were used to synthesize gold nanoparticles on the surface in water and toluene. The sizes of Au particles, synthesized in water and toluene, were 8 and 25nm, respectively. The Au particles on the PDMAEMA side chains reduced surface zeta potential from -10.36 to -16.98 mV, indicateing the Au particles carried negative charges which is induced by R4N+ groups.

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