由基因重組大腸桿菌所製備的非水溶性多精胺酸聚天門冬胺酸，結構上以天門冬胺酸為主鏈，精胺酸與離胺酸為側鏈所組成，目前已被證實是一種溫敏性高分子，具有上限臨界溶解溫度 (upper critical solution temperature, UCST)，透過溫度調控而使其在水溶液中型態產生變化，應用於基因轉染。
本研究利用1-乙基-(3-二甲基氨基丙基)碳二亞胺 (EDC) 和N-羥基琥珀醯亞胺 (NHS) 作為羧基活化劑，使具有羧基的多精胺酸聚天門冬胺酸與聚乙烯亞胺上的胺基形成共價鍵鍵結，接枝物保留良好的生物相容性和溫度敏感性特點作為基因載體。
接枝物利用溫度敏感性和靜電作用的特性與球狀金奈米和DNA結合成複合體，由細胞的內吞作用攝入至細胞中，並以近紅外光照射，引發局部升溫，刺激DNA釋放，利用調整紅外光強度和照射時間可達到最佳的轉染效率。透過流式細胞儀和β-galactosidase基因表現分析，確認含金奈米的複合體所引發的光熱效應可以提高基因的表現量。證實此複合體在基因傳遞方面具有相當的潛力。

The insoluble multi- L-arginyl-poly-L -aspartate (MAPA) produced by recombinant Escherichia coli is composed of aspartate as the main chain with arginine and lysine as the side chains. It has been shown to be a thermo-responsive polymer with upper critical solution temperature (UCST) property, By thermal control to change its form in aqueous solution, it can be expected to be used in gene transfection.
In this study, 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) were used as carboxyl activation agents to covalently bond the carboxyl-functionalized MAPA with polyethyleneimine. The cross-linked polymer retains good biocompatibility and thermo-responsiveness.
Through thermo-responsive and electrostatic interactions, MAPA forms complexes with spherical gold nanoparticles and DNA, and the complex can enter cells through endocytosis. Then, using infrared light irradiation to induce localized heating could stimulate DNA release. Adjusting the intensity and duration of the infrared light irradiation can achieve high transfection efficiency as analyzed by flow cytometry and MUG assay. It has been shown that the photothermal property of the complexes containing gold nanoparticle-containing can increase the expression of genes. The delivery system shows potential in gene transfection.

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