本研究分為三個部分，第一部份為微影製程的圖案化轉印，以聚二甲基矽氧烷（Polydimethylsiloxane，PDMS）為材料，注入不同圖案化的矽晶圓，加熱使其固化，當聚二甲基矽氧烷從晶圓上移除時，即使是最小的微流道設計細節也會印在二甲基矽氧烷的基板上。根據微影製程中光罩的設計，共製備了五種圖案，各別是1um、1.5um、2um、3um四種線寬以及洞型光阻；經過轉印後，成功翻印出四種線寬與柱型結構。。
第二部分為高分子刷製備。包括表面親水處理、合成矽烷類自組裝層、固定聚合起始劑，再藉由原子轉移自由基聚合法(Atom transfer radical polymerization，ATRP)進行表面起始聚合，製備聚甲基丙烯酸（Poly(methacrylic acid)，PMAA）高分子刷，並以原子力顯微鏡(Atomic Force Microscope，AFM)及場發射式掃瞄式電子顯微鏡(Field Emission Gun Scanning Electron Microscopy，FEGSEM)進行形貌分析。
本研究第三部分以EDC/NHS反應將PMAA之Carboxylic acid活化，固定Streptavidin與上皮細胞黏附分子抗體(Anti-Epithelial Cellular Adhesion molecule，Anti-EpCAM)，偵測大腸癌臨床病患血液中的循環腫瘤細胞(Circulating tumor cells, CTCs)。

In this study, we divide into three parts, the first one we using PDMS（Polydimethylsiloxane）to transfer the pattern which on Silica wafer. Inject PDMS into different pattern and heat it led to harden .When removed even the smallest of details is left imprinted in the PDMS. According to the mask design, we fabricate five patterns,1um,1.5um,2um,3um line with the different widths and ordered hole array.
The second part was the fabrication of polymer brush on PDMS, including surface hydrophilic modification, the synthesis of the silane self assembly monolayer (SAM), the immobilization of the initiator of polymerization, and the polymerization of Poly(methacrylate acid) brushes (PMAA) initiated from the surface of silicon wafer via atom transfer radical polymerization (ATRP). Morphology analyzing with AFM(Atomic Force Microscope) and FEGSEM (Field Emission Gun Scanning Electron Microscopy).
The last part, activate the Carboxylic acid of PMAA by EDC/NHS reaction and immobilize Streptavidin and Anti-EpCAM (Anti-Epithelial Cellular Adhesion molecule).Detect the CTCs(Circulating tumor cells) in clinical colon cancer patient blood.

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