研究生: |
劉煒辰 Wei-Chen Liu |
---|---|
論文名稱: |
乳清蛋白製備類澱粉蛋白奈米纖維膜及其對花青素吸附與奈米銀過濾之研究 Amyloid protein nanofibrils membrane prepared from whey protein isolate for adsorption of butterfly pea flower extract and filtration of silver nanoparticles |
指導教授: |
李振綱
Cheng-Kang Lee |
口試委員: |
王勝仕
Sheng-Shih Wang 蔡伸隆 Shen-Long Tsai |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2017 |
畢業學年度: | 105 |
語文別: | 中文 |
論文頁數: | 83 |
中文關鍵詞: | 類澱粉蛋白 、乳清蛋白 、蝶豆花 、奈米顆粒 、奈米纖維 |
外文關鍵詞: | Amyloid protein, whey protein, butterfly pea, nanoparticle, nanofibrils |
相關次數: | 點閱:203 下載:1 |
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類澱粉蛋白奈米纖維(Amyloid protein nanofibrils),是在特定的變性條件下某些蛋白質的二級結構β-sheets會自組裝(self-assembling)所形成的不可溶的蛋白質奈米纖維。本論文第一部分是研究直接利用乳清蛋白(Whey protein isolate)作為類澱粉蛋白奈米纖維之源料,乳清蛋白含有大量的β-Lactoglobulin,可形成類澱粉蛋白奈米纖維,在pH 2及80°C下反應20 小時,經由蛋白質電泳分析可以得知乳清蛋白中β-Lactoglobulin會隨著反應的時間而逐漸減少,而由螢光分析及AFM表面結構觀察,溶液中可得到直徑為15 nm,長度達2 μm之奈米纖維;第二部分則是研究將類澱粉蛋白奈米纖維的回收並固定化於孔徑為0.2 μm之醋酸纖維膜上,此類澱粉蛋白奈米纖維所形成的濾膜可用於天然花青素之吸附濃縮,將濃度為4 mg/mL之花青素溶液濃縮約5倍,在以1,1-二苯基-2-三硝基苯肼(DPPH)抗氧化分析其抗氧化性,抗氧化率可從花青素溶液之75 %提升花青素膜之85 %。此類澱粉蛋白奈米纖維膜可用於奈米銀顆粒之過濾回收,可將顆粒大小為31 nm之奈米銀過濾回收達96 %,此含有奈米銀之纖維濾膜,亦有觸媒反應之活性,在反應30分鐘後可將4-nitrophenol還原成4-aminophenol。
Amyloids are aggregated proteins that self-assembled into fibrils. The proteins or polypeptides generally form β-sheet structure under a denaturation condition before aggregate into long fiber. β-Lactoglobulin is one of often used proteins for amyloid fibrils generation. Whey protein isolate (WPI) has rich content of β-Lactoglobulin was found in this study can be used directly for the preparation of amyloid fibrils under the condition of pH 2 and heating to 80°C for 20 h. The as-prepared fibrils have an averaged diameter of 15 nm and length of more than 2 μm as observed by AFM. The zeta potential of the fibrils was measured to be +12.9 mV at pH 2 and became zero charge at pH 5.2. The amyloid nanofibrils prepared from WPI could be easily fabricated into membrane by filtering the amyloids suspension using 0.2 μm cellulose acetate microfiltration membrane. Clitoria ternatea (butterfly pea flower) extract that has rich content of anthocyanin was easily concentrated by filtering through the WPI amyloid fibrils membrane. The anthocyanin collected on the membrane exhibited strong antioxidant activity as measured by DPPH assay. The WPI nanofibrils membrane could also work as a nanofiltration membrane for the recovery of silver nanoparticles. Silver nanoparticle (AgNP) prepared by citrate reduction method with particle size about 31 nm could be very effectively collected by the nonofibrils membrane that 96.8 % of AgNP was recovered.
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