台灣地質多變(潛因)與氣候變遷(誘因)引致國土危脆，不惟大地防災工程刻不容緩，且基建開發之地盤改良與基礎、邊坡穩定等工程需求日殷，本研究有別於習見之地盤改良如物理式地錨及化學式灌漿等工程技術，改用於存在土壤或海洋中之微生物，先以仿生式之微生物誘導碳酸鈣沉澱(MICP)技術，提升粒狀土壤之膠結與降低其滲透；再由自然岩土之矽質膠結優於鈣質之力學行為，創新使用矽藻進行微生物式矽化膠結(MISP)應用於大地工程改良。透過靜態直剪、單壓試驗與動態邊坡振動模型；同時搭配非破壞式-物理性超音波脈衝(UP)、電子顯微鏡(SEM)、化學性能量色散光譜儀(EDS)，以驗證其改良成效。
本研究除進行鈣化、矽化改良之外，並將生物式鈣-矽化機制嘗試混合，以期產生新的改良可行性，以相對密度60%之砂土改良72小時後，Bio 1凝聚力可提升 0.71 kgf/cm2、Bio 2可提升0.4 kgf/cm2、Bio Mix可提升0.52 kgf/cm2、Bio Mix(no silaffin)可提升0.44 kgf/cm2；於抗壓強度則可從原先無法自立至Bio 1可提升 0.8 kgf/cm2、Bio 2可提升0.3 kgf/cm2、Bio Mix可提升0.55 kgf/cm2、Bio Mix(no silaffin)可提升0.43 kgf/cm2；而水力傳導係數則可由未改良前8 x 10-3 cm/sec下降至Bio 1的5.8 x 10-3 cm/sec、Bio 2的1 x 10-3 cm/sec、Bio Mix的7 x 10-4 cm/sec、Bio Mix(no silaffin) 的8 x 10-4 cm/sec；於剪力波速中可於72小時後Bio 1提升至596 m/s、Bio 2提升至470 m/s、Bio Mix提升至527 m/s、Bio Mix(no silaffin)提升至497 m/s。並將粒狀土壤成功改良轉移至黏性土壤中，透過電滲工法輔助將生物式改良液導入，以初始含水量80%之高嶺土為例，經馴養72小時後，抗壓強度Bio 1提升至3.84 kgf/cm2、Bio 2提升至2.02 kgf/cm2、Bio Mix提升至3.35 kgf/cm2、Bio Mix(no silaffin)提升至2.33 kgf/cm2；剪力波速部分，Bio 1提升至803 m/s、Bio 2提升至669 m/s、Bio Mix提升至775 m/s、Bio Mix(silaffin)提升至718 m/s。無論是生物式鈣化或矽化，抑或是兩者混合使用，皆可相較於未改良之試體有顯著的提升。

Due to the diversification of Taiwan’s geology (potential cause) and climate change (inducing cause) have caused the soil to be fragile. Not only the earth’s disaster prevention project urgent, but also the demand for infrastructure development, including soil geology improvement and foundation, slope stability and other projects are increasing. This research is different from the conventional ones, which carry out soil geology improvement engineering technologies by physical ground anchors and chemical grouting, are used for microorganisms in the soil or ocean. First, biomimetic microbial induced calcium carbonate precipitation (MICP) technology is used to improve the cementation of granular soil and reduce its penetration ; Second, the mechanical behavior of siliceous cementation of natural rock and soil is better than that of calcium, and the innovative use of diatoms for microbial silicified cementation (MISP) is applied to geotechnical engineering improvement. By proceeding static direct shear test, uniaxial compression test and dynamic slope vibration model, with non-destructive-physical ultrasonic pulse (UP) , electron microscope (SEM) , chemical energy dispersive spectrometer (EDS) to verify the improvement effect at the same time.
Apart from improving calcification and silicification, this study tried to mix the biological calcium-silicification mechanism in order to produce new improvement feasibility. After 72 hours of improvement of sand with a relative density of 60%, Bio 1 cohesion can increase by 0.71 kgf /cm2, Bio 2 can increase 0.4 kgf/cm2, Bio Mix can increase 0.52 kgf/cm2, Bio Mix (no silaffin) can increase 0.44 kgf/cm2 ; the compressive strength can be increased from the original inability to support itself to Bio 1 can increase 0.8 kgf/cm2, Bio 2 can increase 0.3 kgf/cm2, Bio Mix can increase 0.55 kgf/cm2, Bio Mix (no silaffin) can increase 0.43 kgf/cm2 ; and the hydraulic conductivity can be increased from 8 x 10-3 cm/sec before the improvement drops to 5.8 x 10-3 cm/sec for Bio 1, 1 x 10-3 cm/sec for Bio 2, 7 x 10-4 cm/sec for Bio Mix, 8 x 10-4 cm/sec for Bio Mix (no silaffin); in shear wave velocity, Bio 1 can be increased to 596 m/s, Bio 2 to 470 m/s, Bio Mix to 527 m/s, Bio Mix (no silaffin) after 72 hours to 497 m/s. The granular soil was successfully improved and transferred to the cohesive soil, and the biological improvement solution was flow into by the electro-osmosis method. Taking kaolin with an initial water content of 80% as an example, after 72 hours of domesticating, the compressive strength Bio 1 increased to 3.84 kgf/cm2, Bio 2 increased to 2.02 kgf/cm2, Bio Mix increased to 3.35 kgf/cm2, Bio Mix (no silaffin) increased to 2.33 kgf/cm2; and the part of shear wave velocity, Bio 1 increased to 803 m/s, Bio 2 increased to 669 m/s, Bio Mix increased to 775 m/s, and Bio Mix (silaffin) increased to 718 m/s. No matter it is biological calcification or silicification, or hybrid of the two, it can be significantly improved compared to the unimproved specimen.

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