糖化纈草胺酸 (fructosyl valine, FV) 為糖化血紅素 (HbA1c) 的末端糖化官能基，糖化血紅素在血液中濃度可以反應出體內二至三個月的平均血糖濃度。因此糖化纈草胺酸之濃度可作為糖尿病之指標。
　　本研究是以電化學方法，偵測由糖化胺基酸氧化酶 (fructosyl amino acid oxidase，FAO) 氧化糖化纈草胺酸，所產生之過氧化氫。在旋轉式碳電極表面塗佈一層含鉑-多層壁奈米碳管觸媒催化過氧化氫，以增強電流訊號，於施加電位0.6V時，偵測過氧化氫之線性範圍為0.00125-6.05 mM，靈敏度為1027.34 μA mM-1 cm-2，最低偵測極限為0.00125 mM；在觸媒層上再固定化一層FAO酵素固定層，此層主要是以覆有約10 nm奈米金之奈米纖維素為載體以利FAO酵素之固定。製作出高靈敏度糖化血紅素感測器，其對糖化纈草酸靈敏度為135.44 μA mM-1 cm-2 ，線性範圍為0.01-1.92 mM，最低偵測極限為0.01 mM。
將觸媒及酵素修飾層移至微型感測器電極上 (工作電極面積為0.0018cm2)，其過氧化氫靈敏度為177.02 μA mM-1 cm-2，糖化纈草胺酸靈敏度為47.65μA mM-1 cm-2，線性範圍0.05-1.19 mM，且測試時間只需5秒，成功製備出偵測快速且具高靈敏度之糖化血紅素感測器。

Fructosyl valine (FV) is the glycated functional end group of glycosylated hemoglobin (HbA1c). In human body, the level of HbA1c can reflect the average blood sugar level within the recent 2 to 3 months. Consequently, the measurement of the concentration of HbA1c can be used to obtain the concentration of sugar which is important for the long-term control of the glycemic state of diabetic patients.
　　 In this study, FV was catalyzed by fructosyl amino oxidase (FAO) to produce hydrogen peroxide, which could be detected electrochemically. Platinum-Carbon nano tube (Pt-CNT) catalyst was coated on glassy carbon rotating disk electrode (GCRDE) to enhance the current signal by catalyzing hydrogen peroxide. The working potential was fixed at +0.25 V versus the reference electrode(Ag/AgCl), the range of hydrogen peroxide detection ranged from 0.00125 to 6.05 mM, with the sensitivity of 1027.34 μA mM-1 cm-2,and the limit of detection (LOD) at 0.00125 mM. FAO was coated on 10 nm Au particles of Au-BC and further coated onto the catalyst layer to obtain HbA1c sensor. The results showed that the fabricated HbA1c sensor possessed sensitivity of 135.44 μA mM-1 cm-2 with linear detection range between 0.01 and 1.92 mM, and with the limit of detection of 0.01 mM.
　　 By applying the optimized experimental parameters on the disposable electrodes (with working area of 0.0018 cm2), the results exhibited sensitivity of 177.02 μA mM-1 cm-2 and 47.65μA mM-1 cm-2 for detecting H2O2 and FV in the range of 0.05 and 1.19 mM, respectively. A fast response time of less than 5 seconds was obtained. In conclusion, we demonstrated that a highly sensitive HbA1c sensor was successfully prepared not only on glassy carbon electrode but also on portable microelectrode which can be applied for diabetes patients.

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