本研究探討靜電紡絲製程中電場電壓、溶液濃度、距離等條件，以明膠（gelatin）為溶質，甲酸、乙醇水溶液為溶劑所構成的溶液製備具備奈米結構之不織布薄膜，以得到各種不同纖維直徑的薄膜。由實驗結果，可得知最佳條件為濃度15%，噴絲距離15cm，電壓20kV，可得纖維之平均尺寸為50~100nm。所得之明膠不織布，用戊二醛（glutaraldehyde）交聯，同時量測活化部分凝血酶時間(APTT)、血小板凝集程度來評估凝血性質。此外明膠不織布在細胞植入效率和增生性測試，以CRL 1927腎臟間質細胞體外培養測試，結果發現明膠不織布會有很高的細胞植入效率且能促進腎臟間質細胞的增生。

This research discusses the effect of the electrospinning conditions including voltage, concentration, and distance on the thermal and biological properties of gelatin fibers. The spinning dope consisted of gelatin, formic acid, ethanol, and water as solvent. The results show that the gelatin fiber spun with 20kV from the 15% dope solution exhibited better nanostructure. The highest uniformity in the fiber diameter was attained when the distance was 15cm. After crosslinking with glutaraldehyde, the nonwoven exhibited higher thermal stability. In addition, gelatin non-woven exhibits a higher cell seeding efficiency than the control (TCPS) and also promotes CRL 1927 proliferation.

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