靜電紡絲蠶絲蛋白/二氧化鈦奈米纖維薄膜作為生醫敷料之探討

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研究生：	徐其全 Chi-Chuan Hsu
論文名稱：	靜電紡絲蠶絲蛋白/二氧化鈦奈米纖維薄膜作為生醫敷料之探討 Preparation and characterization of electrospun silk fibroin/TiO2 naofibers for biomedical applications
指導教授：	楊銘乾 Ming-Chien Yang
口試委員:	王大銘 Da-Ming Wang 蘇清淵 Ching-Yuan Su
學位類別：	碩士 Master
系所名稱：	工程學院 - 材料科學與工程系 Department of Materials Science and Engineering
論文出版年：	2010
畢業學年度：	98
語文別：	中文
論文頁數：	92
中文關鍵詞：	靜電紡絲、蠶絲蛋白、二氧化鈦、創傷敷料
外文關鍵詞：	Electrospinning, Silk fibroin(SF), TiO2, Wound
相關次數：	點閱：383 下載：5
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本研究以靜電紡絲製備蠶絲蛋白奈米纖維薄膜，並探討其溶液濃度對成絲之影響，以製作最佳化的蠶絲蛋白纖維薄膜。而後混摻不同濃度之二氧化鈦奈米顆粒，在最佳參數條件下製成蠶絲蛋白/二氧化鈦奈米纖維薄膜。本實驗探討奈米纖維的製備效果與添加二氧化鈦奈米顆粒後的基本物理性質、血液與生物相容性。
由SEM得知蠶絲蛋白的濃度達10%(w/v)時，出現完整的不織布狀奈米級纖維，平均尺寸約386±73 nm ；添加二氧化鈦後也有良好的成絲性。FTIR光譜中出現Silk I、SilkII分子構型的改變。亞甲基藍溶液經過24小時的光觸媒分解其濃度減低近50%。材料熱穩定性和表面粗糙度隨著二氧化鈦濃度增加而上升。蠶絲蛋白/二氧化鈦薄膜具有比市售敷料較佳的含水率與水蒸氣穿透率。血液常規測試與細胞毒性並無特殊異常。觀察纖維母細胞L929在蠶絲蛋白/二氧化鈦薄膜上的貼附有明顯地增加。抗菌性測試也顯示出對於大腸桿菌(E.coli)有明顯的抑菌性。
總括而言，本實驗製備的蠶絲蛋白/二氧化鈦奈米纖維薄膜具有潛力應用於組織工程之創傷敷料相關研究。

Using electrospinning to fabricate silk fibroin (SF) nanofiber film was investigated, thus blending different weight ratio of TiO2 with SF solution fabricates SF/TiO2 hybrid nanofibers with optimizative parameters. In this study, we investigated the preparation of nanofibers, and the physical properties, biocompatibility after blending with TiO2 nanoparticle.
Scanning electron microscope (SEM) revealed that the SF nanofibers was formed with average diameters 386±73 nm when the concentration of SF was up to 10 %(w/v). The SF/TiO2 nanofibers film was also successfully prepared. FTIR identified the conversion of the random coil (Silk I) conformation of SF into β-sheet (Silk II) by treating with acetone. The maximum photocatalytic decomposition of methylene blue solution was nearly achieved to 50% after 24 hours. Compared with pure SF film, the thermal stability and surface roughness was improved by the blending with TiO2 nanoparticle. The SF/TiO2 nanofibers film provided a better level of equilibrium water content (EWC) and water vapor transmission rate (WVTR) than TegasorbTM (Hydrocolloid dressing). Complete blood count (CBC) and cytotoxicity showed no significantly difference. The SF/TiO2 nanofibers film was found to promote the adhesion, growth and spreading of L929 fibroblasts cells according to a three dimensional network of the nanofibrous structure, and exhibited antibacterial activities against Escherichia coli. In conclusion, the novel nanocomposite mats may be a good candidate as wound dressing for tissue engineering applications.

中文摘要                                                  II
英文摘要                                                  III
誌謝	                                                 IV
目錄                                                      V
圖索引                                                    IX
表索引                                                    XI
第一章 緒論                                               1
  1-1研究背景                                             1
  1-1研究目的                                             3
第二章 文獻回顧                                           4
  2-1靜電紡絲介紹                                         4
    2-1-1電紡基本原理                                     4
    2-1-2電紡裝置架設                                     6
    2-1-3影響電紡之因素                                   7
  2-2生醫材料                                             8
    2-2-1蠶絲蛋白                                         8
    2-2-2蠶絲蛋白結構改性                                13
    2-2-3聚氧乙烯(PEO)                                   17
  2-3光觸媒TiO2之介紹                                    18
  2-4皮膚                                                20
    2-4-1皮膚之功能                                      20
    2-4-2皮膚之構造                                      21
    2-4-3皮膚癒合之機制                                  22
  2-5創傷敷料                                            24
    2-5-1敷料之發展演進                                  24
    2-5-1人工敷料之種類                                  25
第三章 實驗材料與方法                                    29
  3-1實驗藥品                                            29
  3-2實驗儀器                                            30
  3-3實驗流程                                            31
  3-4實驗方法                                            32
    3-4-1蠶絲蛋白/二氧化鈦纖維薄膜製備                   32
      3-4-1-1蠶絲蛋白之純化                              32
      3-4-1-2配置蠶絲蛋白電紡溶液                        33
      3-4-1-3奈米纖維薄膜製備                            34
      3-4-1-4蠶絲蛋白纖維結構改性                        35
    3-4-2紅外光譜分子結構鑑定(FTIR)                      36
    3-4-3光觸媒對有機物之分解性能                        37
    3-4-4分子表面型態分析                                37
      3-4-4-1原子力顯微鏡(AFM)                           37
      3-4-4-2掃描式電子顯微鏡和能量散譜分析(SEM&EDX)     38
    3-4-5材料熱性質分析                                  39
      3-4-5-1熱重分析儀(TGA)                             39
      3-4-5-2熱示差掃描分析儀(DSC)                       39
    3-4-6平衡含水率(EWC)                                 40
    3-4-7水蒸氣穿透速率(WVTR)                            41
    3-4-8血液常規測試(CBC)                               42
    3-4-9生物相容性                                      44
      3-4-9-1細胞培養                                    44
      3-4-9-2細胞毒性測試                                44
      3-4-9-3細胞增生性                                  46
      3-4-9-3抗菌測試                                    48
第四章 結果與討論                                        50
  4-1奈米纖維薄膜製備                                    50
    4-1-1蠶絲蛋白奈米纖維結構形態                        50
    4-1-2蠶絲蛋白/二氧化鈦奈米纖維製備                   55
    4-1-3蠶絲蛋白/二氧化鈦奈米纖維改性                   56
  4-2能量散佈光譜儀分析(EDX)                             58
  4-3原子力顯微鏡(AFM)                                   60
  4-4紅外光光譜儀分析(FTIR)                              63
  4-5光觸媒對有機物之分解性能                            66
  4-6熱重分析儀(TGA)                                     67
  4-7熱示差式掃描分析儀(DSC)                             69
  4-8平衡含水率(EWC)                                     71
  4-9水蒸氣穿透速率(WVTR)                                73
  4-10血液常規測試(CBC)                                  76
  4-11細胞毒性測試                                       78
  4-12細胞增生微型態觀察                                 80
  4-13抗菌測試                                           82
第五章 結論                                              84
第六章 參考文獻                                          85
作者簡介                                                 93

                                

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全文公開日期 2015/07/05 (校內網路)
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