石墨烯由於其優良的導熱、導電及機械性質，受到極大的關注，目前此種材料在電子及材料領域已有相關應用，近年來在生醫領域的研究多直接將石墨烯用於細胞培養等體外生物實驗，而目前僅有少數石墨烯複合材料相關研究。三維多孔性可降解生醫支架廣泛應用於骨組織工程的修復與再生，此支架的主要功能為提供可讓細胞附著、增生的結構及暫時支撐骨頭受損部位，然而以高分子為基材之支架其有機械強度不佳、疏水性質…等缺點。本論文選用生醫高分子材料聚己內酯(Poly-ε-caprolactone, PCL)為基材，添加石墨烯(Graphene)粉末，以氯化鈉(Sodium Chloride, NaCl)為致孔劑，經由溶液澆鑄/粒子析出法製備生醫多孔性支架。
本論文將不同比例之石墨烯(Graphene)粉末混入生醫高分子材料(PCL)，針對製備之支架探討其表面形貌、機械強度(壓縮試驗)及物理特性(親疏水性測試、孔隙率)，並以降解實驗測試支架之重量損失及pH值變化。生物相容性研究部分，將類骨母細胞(MG63)培養於支架上，並且進行細胞存活率分析(MTT assay)及鹼性磷酸酶檢測(ALP assay)。

Graphene has attracted many attentions because of its excellent thermal, electrical and mechanical properties. Graphene-based materials have been applied in electrical and material fields. In recent years, graphene was directly used in cell culture in a number of studies. Researches on graphene composites are in the minority. Three -dimensional porous biodegradable polymer scaffolds have been widely used for tissue engineering of bone repair or regeneration. The primary function of scaffolds is to provide structure support for the cells adhesion and proliferation. However, scaffolds based on polymer material have some drawbacks such as poor mechanical property, hydrophobicity, and so on. In this study, porous scaffolds were fabricated using Poly-ε-caprolactone (PCL) and graphene platelets by the solution casting/particulate leaching method.
Graphene platelets were blended with PCL in this study. Mechanical properties, physical properties (porosity, contact angle) of fabricated scaffolds were investigated first. Subsequently, the weight loss and pH values variation of PCL/graphene scaffolds were also obtained by degradation test. Biocompatibilities of PCL/graphene were observed by seeding osteoblast-like cells (MG-63) in PCL/graphene scaffolds. Investigations of biocompatibility were undertaken by 3-[4,5-dimethylthiahiazo-2-y1] -2,4-diphenytetrazolium bromide (MTT) assay and alkaline phosphatase (ALP) assay.

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