於本研究中我們製備了四氧化三鐵超順磁性奈米粒子，並運用溶膠凝膠法(sol-gel process)合成核殼結構(core-shell)之Fe3O4@SiO2奈米粒子，予以改質後，將Protien G鍵結於奈米粒子表面，以增加抗體的順向性；接著，我們於其表面同時接上抗人體血清蛋白(anti-ALB)及碲化鎘(CdTe)，並透過CdTe之螢光遮蔽效應用以檢測人體血清蛋白(ALB)，最終利用圖案化鎳-磁性模具作為磁微影遮罩使奈米粒子產生圖案化，並應用於光學免疫測試。
首先我們以超導量子干涉磁量儀(SQUID)及穿透式電子顯微鏡(TEM)證實合成之奈米粒子為核殼結構且具有超順磁性，並經由螢光光譜(FP)以及酵素連結免疫吸附分析法(ELISA)探討具有抗體順向性之奈米粒子的螢光遮蔽效率及抓取抗原之能力，發現有鍵結Protein G之奈米粒子不僅抓取抗原的能力高達2.2614μg/mg，且螢光遮蔽效率更提升至59.40%，已可於肉眼明顯觀察其亮度之變化。
接著利用磁力顯微鏡(MFM)證實製備出線寬為1μm、3μm及洞寬為500nm之鎳-磁性模具，並經由光學顯微鏡(OM)及雷射共軛焦顯微鏡(CLSM)證實利用線寬3μm鎳-磁性模具可製成最小尺寸且完整之圖案化感測器，並成功應用於光學免疫測試。

In this study, we fabricated superparamagnetism nanoparticles and core-shell structures comprising Fe3O4 and SiO2 as the core and shell components, respectively. After surface modified with NH2 group(MNPs), Protein G was bound to the surface of MNPs for increasing the orientation of anti-ALB. Then, anti-ALB and CdTe were bound to MNP@Protein G and we detected ALB by the shelter effect of CdTe. In addition, we patterned composite nanoparticles by Ni-mask with magnetolithography for immunosorbent assay.
Superconducting Quantum Interference Device Magnetometer (SQUID) and Transmission Electronmicro-scope(TEM) revealed superparamagnetism and the core/shell structure. Then we investigated the orientation of anti-ALB with the sheltered efficiency and the ability of binding antigen by Spectrofluorometer(FP) and Enzyme-linked immunosorbent assay (ELISA). We also demonstrated the amounts of ALB and the sheltered efficiency were achieved to 2.2614μg/mg and raised to 59.40%, respectively. We could observe the difference of the fluorescent intensity significantly.
Finally, the Ni-masks were successful fabricated with the line-width (1μm and 3μm) and hole-width (500nm). Then, we demonstrated we used the Ni-mask of line-width 3μm to show the smallest scale and complete pattern for optical immunosorbent assay by Optical Microscope(OM) and Confocal Spectral Microscope Imaging System(CLSM).

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