由於軟骨組織自體修復的諸多限制與高齡化社會的來臨，關節軟骨缺損的治療研究近年來逐漸受到重視。

　　藍藻蛋白為非核糖體合成蛋白質，具備良好的生物相容性與降解性，結構帶正電有助於細胞貼附。葡聚糖為天然多醣，具備良好的生物相容性與降解性，另外還具備水溶性與保水性，其表面不帶電，不利於細胞貼附。過去在組織再生的研究中曾用來作為醣胺聚醣的類似物與有助於細胞貼附的高分子結合，模擬基質組成進行細胞培養。

　　本研究從「仿生」概念出發，將兩種材料以不同比例結合，針對豬軟骨細胞於藍藻蛋白-葡聚糖接枝薄膜的生長進行探討，期待藍藻蛋白-葡聚糖接枝薄膜能作為軟骨組織工程研究中另一個可行的基材。

　　藍藻蛋白-葡聚糖接枝產物利用比色法、分子量鑑定與元素分析方法得到具體的接枝程度，以FT-IR觀察接枝前後官能基變化，並測試溶解度。經戊二醛交聯而成的藍藻蛋白-葡聚糖接枝薄膜以螢光染色法確認交聯效果。並觀察不同時間點的膨潤度。將CHO cell培養於薄膜進行初步的細胞生長測試，再將不同代數的豬軟骨細胞培養於薄膜。以MTT assay、Bradford protein assay與DNA quantitation三種方法測試豬軟骨細胞於薄膜上的增生能力，以Blyscan assay測試醣胺聚醣合成效率，並以qRT-PCR測試特定mRNA的表現。
結果顯示藍藻蛋白接枝葡聚糖後可改善溶解度的限制。而形成藍藻蛋白-葡聚糖接枝薄膜後，膨潤度隨葡聚糖比例的提高而增加。藍藻蛋白改善了在軟骨組織自癒過程中醣類不利於細胞貼附的缺點，提高細胞貼附與增生能力，同時適量的藍藻蛋白可維持葡聚糖對於豬軟骨細胞的生長趨向體內生長狀態的助益，對於較高代數的軟骨細胞依舊有不錯的成效。

　　綜上所述，藍藻蛋白接枝葡聚糖薄膜可維持軟骨細胞一定的增生能力，有效延長豬軟骨細胞體外培養維持機能的時間，甚至幫助高代數的豬軟骨細胞恢復分化能力。

　　The studies of treatments for articular cartilage defects have received lots of attentions due to the limitations of self-repair abilities of articular cartilage.

　　Cyanophycin, a non-ribosomal synthesis protein, has good biocompatibility and biodegradability, and its structure is positively charged which is helpful for cell adhesion. Dextran, a kind of natural polysaccharides, also has good biocompatibility and biodegradability in addition to good water solubility and good capability for water retention. However, it has no charges for cells to adhere onto. In the previous studies regarding tissue regeneration, dextran has been used as a substitute for glycosaminoglycan, and combined with other cell-adhesion polymers to mimic the structure of extracellular matrix for cell growth. This study is based on “biomimetic”, to combine the above two biomaterials, and focused on assessing the growth of porcine chondrocytes on the films of cyanophycin-dextran conjugate.

　　The grafting ratios of cyanophycin-dextran conjugate were determined by colorimetry, gel permeation chromatography (GPC) and elemental analysis (EA), and Fourier transform infrared spectroscopy (FT-IR) was used to analyze the change in the functional groups of the conjugates. The films of cyanophycin-dextran conjugate were cross-linked with glutaraldehyde, and the cross-linking results were observed under a fluorescence microscopy after fluorescence staining. The swelling ratios were tested at different time intervals. CHO cells were grown on the films as a primary test for cell viability, and then two different passages of porcine chondrocytes were further grown on the films. The cell viability was monitored by MTT assay, Bradford protein assay and DNA quantitation. The glycosaminoglycan contents were examined by Blyscan assay, and the specific mRNA expressions were measured by qRT-PCR.

　　The results showed that the grafting of dextran enhanced the water solubility of cyanophycin, and the swelling ratios of the films increased with dextran grafting. On the other hand, the grafting of cyanophycin improved cell-adhesion on the polysaccharides, one of the major limitations of cartilage self-repair. Meanwhile, moderate contents of cyanophycin could maintain the advantages of dextran which provided a growth condition for chondrocytes similar to an in vivo one. Moreover, the films could be helpful for aging chondrocytes.

　　In conclusion, the films of cyanophycin-dextran conjugate could maintain cell viability and delay chondrocyte dedifferentiation, and could help aging chondrocytes regenerate.

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