本論文主要以合成螢光CuInZnS/ZnS量子點以及量子點結合具有光熱/光動力效果的異質結構奈米晶體材料，在材料的分析和應用上分成兩部分說明

第一部份: 針對螢光CuInZnS/ZnS量子點提出一個簡單且快速的製備方式。藉由水熱合成法並搭配雙穩定劑可直接合成出高螢光量子產率的水溶性量子點。 後續利用交聯反應對量子點表面嫁接苯硼酸分子，研究發現苯硼酸分子可以專一性地對Hela子宮頸癌細胞呈現螢光影像。另外，Hela子宮頸癌細胞和斑馬魚的毒性測試皆指出CuInZnS/ZnS量子點為低毒性奈米材料，期待後續可作為醫學上的應用。

第二部份: 本實驗發展出以牛血清蛋白將有機相的Cu2S/CIS異質結構晶體進行相轉換到水溶液相，同時包覆在Cu2S/CIS表面的牛血清蛋白又能螯合Au螢光團簇形成新型態的奈米複合物。利用Cu2S/CIS異質結構光熱(photothermal)的性能搭配Au團簇之螢光和光動力(photodynamic)性能，使得本材料兼具光熱/光動力治療以及細胞標靶螢光影像等多功能性的奈米材料。

In the frist part of this study, dual-stabilizer-capped CuInZnS/ZnS (CIZS/ZnS) quantum dots (QDs) in aqueous media via hydrothermal routes were synthesized. The surface of CIZS/ZnS QDs effectively coated with dual stabilizer, resulting higher quantum yield and carboxylic groups that conjugate with various materals. The dual-stabilizer-capped CIZS/ZnS QDs were conjugated with 3-aminophenylboronic acid (APBA), termed CIZS/ZnS@APBA, for Hela tumor cell labeling. In vitro and in vivo assessments, using Hela cells and zebrafish embryos respectively, revealed that CIZS/ZnS@APBA nanoprobes exhibit low cytotoxicity. Confocal scanning laser imaging demonstrated that nanoprobes can be efficiently internalized and are localized in the cytoplasm of Hela cells. Analysis of ruslts reveals that potential use of CIZS/ZnS@APBA as a safe and effective targeted probe for in vivo imaging of cancer.
The second part is the preparation multifunctional Au-Cu2S/CIS nanocomposite, which could be able to apply in fluorescent imaging and photothermal/photodynamic therapy. The fluorescent imaging and acute toxic response of Au-Cu2S/CIS nanocomposite were checked by using Hela tumor cell. Furthermore, the generation of ROS in Hela tumor cells induced by Au-Cu2S/CIS before and after visible light irradiation were checked. The analysis result reveal that Au-Cu2S/CIS nanocomposite show photothermal and photodynamic effect and fluorescent imaging probes for specific cancer diagnosis.

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