使用載體包覆藥物改善藥物傳輸系統，提升藥物的療效並降低副作用的方法已被廣泛使用。相較於合成聚合物，天然聚合物有較佳的生物相容性和生物可降解性，因此可作為載體的材料。海藻酸是一種天然聚合物，在食品工業可作為乳化劑或增稠劑，它可以和二價陽離子結合形成具有網狀結構的凝膠，因此做為載體來使用也十分合適。有關於製備海藻酸凝膠顆粒的方法已被大量研究，然而不同製備方法都有其應用範圍的限制，故不斷的對其進行研究是有必要的。因此本研究選擇較少人使用的二流體噴嘴來製備海藻酸凝膠顆粒，藉由分析粒徑大小和外觀型態，評估合適的製備參數，並將其用來做為包覆二甲雙胍和順鉑的載體，評估其包覆率和生物相容性。
研究結果顯示，以二流體噴嘴製備海藻酸凝膠顆粒，較為合適的參數為：海 藻酸濃度至少1.5%，噴霧壓力至少4.5 kg/cm2，氯化鈣濃度為1%。由此條件製 備而成的顆粒大小為12-16μm；包覆藥物的顆粒實驗顯示，氯化鈣濃度的提高可 以增加藥物的包覆率；細胞實驗的結果顯示，所製備的顆粒生物相容性良好，藥 物亦能夠由顆粒中釋出，故將此顆粒用於包覆藥物改善藥物的傳輸系統是一可行的方法。

Drug delivery systems can improve drug efficacy and reduce the side effects which are widely used now. I made our drug delivery system by using drug carriers to encapsulate the drugs. Natural polymers can be used as carrier material because natural polymers have better biocompatibility and biodegradability than synthetic polymers. Alginate is a natural polymer, widely used in the food industry as an emulsifier or thickener. It can be binded with divalent cations and then formed a gel which has a network structure. Continuing the research in making alginate gel particles is necessary because there are different limitations in different preparation methods. There are few journals related to alginate gel particles which are used by two-fluid nozzle. Therefore, I chose two-fluid nozzle as an object of study. The particles were analyzed to detect particle size and appearance of patterns, assess suitable preparation parameters. It is used as a carrier to encapsulate metformin and cisplatin to evaluate encapsulate efficiency and biocompatibility.
The suitable parameters of the alginate gel particles prepared by two-fluid nozzle are: alginate concentration of at least 1.5%, spray pressure of at least 4.5 kg/cm2, calcium chloride concentration needs to be 1%, and particle size is 12-16μm. Drug encapsulation with particles experiment shows that the higher concentration of calcium chloride can increase encapsulation of drug. Results of cell experiments showed particles produced good biocompatibility, can also release the drug from the particles, so this particle for encapsulate drugs to improve drug delivery system is a feasible method.

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