近幾年來科技進步，對於口腔癌的診斷與治療大幅成功許多，但是對於後期階段的口腔癌患者來說，存活率僅有20%。預防勝於治療，口腔癌前病變遠遠比口腔癌來的盛行，因此找出一個簡單有效又不具有侵入性的診斷及治療方法極其重要，例如適用於治療淺層癌症之光動力治療。
厚朴酚(Magnolol)來自於中草藥木蘭科植物厚朴樹皮之萃取物，已知其具有抗發炎、抗腫瘤、抗應力以及止瀉的效果。尤其Magnolol在細胞體外實驗以及動物體內實驗都證實了擁有很強的抗氧化能力。但Magnolol因其具疏水、懸浮不佳等特性，因此在臨床醫學的應用上，受到諸多的限制。5-Aminolevulinic Acid(ALA)為一經過美國食品和藥物管理局認可上市後，廣泛應用於光動力治療上的藥物，本身不具生物毒性，針對癌細胞具有標靶的作用。為了改善Magnolol疏水及懸浮不佳等問題，本研究利用高分子PCADK包覆Magnolol製備成微米顆粒(PCADK-Magnolol MPs)與奈米顆粒(PCADK-Magnolol NPs)，透過MTT assay評估顆粒對人類口腔類上皮癌細胞(OEC-M1)之抑制效果，並探討添加5-Aminolevulinic Acid對顆粒載體抑制癌細胞合併療法之效果。
研究結果顯示相對於微米顆粒，奈顆粒(PCADK-Blank NPs)在高濃度下對癌細胞具有一定的毒殺作用，搭載藥物之顆粒載體PCADK-Magnolol NPs對於抑制OEC-M1之能力較PCADK-Magnolol MPs佳。另一方面Magnolol顆粒載體添加5-Aminolevulinic Acid藥物之合併療法對於OEC-M1抑制能力具有較佳的毒殺作用。 

It is easier to treat oral cancer by improvement of technology in recent year. But the survival rate of oral cancer patients in the late-stage is only 20%. Preventing the happening of cytopathy is more important than the treatment. So, we should find a simple way to treat and diagnose without invasion such as photodynamic therapy for superficial carcinoma.
Magnolol, a kind of traditional herbal medicines extracted from the Magnolia, is known for anti-inflammatory, antineoplastic, anti-stress and antidiarrheic. Magnolol has the evidence of strong antioxidant capacity in vitro and in vivo experiment. However, its hydrophobicity and poor suspension property under physiological condition make it have many limitations in clinical medicine. 5-Aminolevulinic Acid (ALA) is one kind of drug used in photodynamic therapy (PDT) popularly and has been approved by US Food and Drug Administration. It has no biotoxicity and can be used as target-drug in cancer treatment. In this study, PCADK polymer was used to encapsulate magnolol into microparticles and nanoparticles to improve the poor hydrophobicity and suspension property of Magnolol. MTT was used to analyze the relative cell viability of oral epidermoid carcinoma cell (OEC-M1) and to estimate the antitumor effect of magnolol on OEC-M1. Furthermore, this study investigates the effect of combination therapy by adding 5-ALA to OEC-M1 cells which were treated with magnolol loaded particles.
The results showed that PCADK-Blank NPs had higher cytotoxicity than PCADK MPs at high concentration condition. The inhibitory effect of PCADK-Magnolol NPs to OEC-M1 was also superior to PCADK-Magnolol MPs at same magnolol concentration. More importantly, the combination therapy of 5-ALA and magnolol loaded particle showed the synergetic inhibitory effects on OEC-M1.

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