聚二甲基矽烷(Polydimethylsiloxane，PDMS)由於具有化學特性、且易加工又具有良好機械特性而被廣用在生物技術中。雖有許多良好的特性，但親水性不佳，目前最廣用於改質PDMS親水性的技術為常壓電漿噴射束(Atmospheric pressure plasma jet，APPJ)，但無法解決的問題是親水性會隨著時間漸漸變回疏水的狀況。為了克服此應用上的障礙，本研究以二次電漿改質法(Two step plasma treatment，TSPT)改質PDMS，進而達到永久親水，同時賦予表面載體功能。TSPT法第一步驟為將原本疏水的PDMS經過常壓電漿預改質，使表面親水化後，以旋轉塗佈法塗上HEMA微球溶液並以低溫去水烘乾；第二步驟在將吸附HEMA微球的PDMS進行第二次電漿改質，使表面聚合而減緩水降解特性。
透過不同的APPJ激發功率(100W/150W/200W) 並加入1% O2/Ar作為主氣體。以水滴接觸角(Water contact angle)與DPPH自由基定量測量APPJ對PDMS之親水性變化。以反射式傅立葉轉換紅外光光譜儀(ATR-FTIR)測定載體薄膜化學特性。以SEM觀察載體微球形貌。後續以生物相容性確定薄膜不具生物毒性、以白蛋白吸附測定表面具有抗蛋白吸附特性、薄膜本身於金黃色葡萄球菌與大腸桿菌終能有效的隔絕細菌生長到薄膜上，具良好的抗菌特性，且載體薄膜於藥物釋放測試中，具有線性釋放之成果。

關鍵字；常壓電漿噴射數(APPJ)、二次電漿改質法(TSPT)、親水性、HEMA微球、藥物釋放特性

Polydimethylsiloxane (PDMS) is the most widely and one of the most versatile material used in the construction of biotechnology. However, the surface of PDMS is naturally hydrophobic limited its use. Atmospheric pressure plasma jet (APPJ) technology has been widely used for modify surface of PDMS. Conversely, the quick hydrophobic recovery is one of the main issues limiting the use of plasma technology. To overcome the problem of hydrophilic aging. HEMA microspheres solution were simultaneously grafted onto PDMS using a two-step plasma treatment (TSPT). The first step of this method: plasma pretreatment of the PDMS, spin-coating HEMA microspheres mixtures solution, and drying. The second step was carried out by plasma polymerization of preabsorbed reactive HEMA monomers on the surface of dried pretreated films.
The PDMS sample were treated at different plasma power (100W/150W/200W) with 1% O2/Ar, while the wettability and ageing effects were study by water contact angle and DPPH radical test. Surface chemical compound were measured by ATR-FTIR which can prove the grafting film had been grafted onto PDMS. It was also observed the biocompatibility by cultured Fibroblast cells (L929), anti protein absorption character by absorbing Bovine serum albumin (BSA), drug delivering property by linear drug releasing and antibacterial character.

Keywords: APPJ, TSPT method, Hydrophilic aging, HEMA micro-carrier, Drug releasing

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