將聚氨酯(Polyurethane)與奈米銀粒子利用靜電紡絲系統下，製備出具有潛力可以治療感染型傷口的敷材。本研究利用多元醇化學還原法製備出奈米銀粒子，分別以不同濃度(0.17 M與0.34 M)與聚氨酯結合，製備出0.17 M與0.34 M Ag/PU nonwoven mats。透過傅立葉轉換紅外線光譜(FT-IR)與X射線光電子能譜儀(XPS)分析下，發現聚氨酯的硬片段與奈米銀粒子產生作用力。在穿透式電子顯微鏡下(TEM)觀察下，發現奈米銀粒子確實包覆於聚氨酯電訪纖維內部。在場發射掃描電子顯微鏡(FE-SEM)拍攝Ag/PU nonwoven mats表面型態，利用ImageJ的DiameterJ影像分析得到其孔隙率均為50.52~53.61%、孔隙面積0.37~0.64 μm2與纖維直徑175.7~260.5 nm。在對金黃色葡萄球菌(Staphylococcus aureus，BCRC10781)的抑菌圈測試(Inhibition zone test)結果中，定性分析出Ag/PU nonwoven mats確實具有抑菌表現。在Agar extraction process測試下，定量分析出Ag/PU nonwoven mats具有98.26~99.84 %的殺菌率。在透氣性測試(BS EN13726-2:2002)方面，Ag/PU nonwoven mats顯示具有1440.67~1664.33 g/m2高度的透氣能力，在吸水性測試(BS EN13726-1:2002)，Ag/PU nonwoven mats具有0.26 g/cm3吸水量，在銀離子釋放測試(Silver-ion release)中，在0~12小時銀離子被大量釋放，而最大釋放量為0.69 μg/mL，持續到44小時都有銀離子被釋放。在細胞毒性測試方面，細胞存活率都在90%以上，顯示最大釋放量並不會產生細胞毒性。在抗發炎能力測試中，發現Ag/PU nonwoven mats具有抗發炎能力。而活體動物實驗中，在小黑鼠(C57BL6/J mice)背部全皮切開手術(1.2 cm×1.2cm)後，進行非感染動物實驗，總共四組(Aquacel、PU、0.17 M與0.34 M)，經過十天，Aquacel組傷口癒合程度21.48 %，PU組為28.51%，0.17 M為42.12%，0.34 M為35.02 %。最後進行感染動物實驗，經過四天，Aquacel組傷口癒合程度22.05%，PU組為57.08%，0.17 M為48.75%，0.34 M為43.72%。在病理切片分析中，膠原蛋白纖維的緻密程度最好為0.17 M。

The study of antimicrobial Polyurethanecro-based dressings, is reported in here. Polyvinylpyrrolidone (PVP) protected silver nanoparticles were produced by chemical reduction method using ethylene glycol as the reducing agents silver nanoparticles. Fourier transform infrared spectroscopy (FT-IR) and high resolution X-ray photoelectron spectrometer (XPS) confirmed the chemical interaction between the hard segments of PU and silver nanoparticles. Transmission Electron microscopes (TEM) indicated that silver nanoparticles is extrapped in/on the nonwoven mats. The morphology and diameter of Ag/PU nonwoven mats are investigated by high resolution field-emission scanning electron microscope (FE-SEM). The Ag/PU nonwoven mats are composed of electrospun fiber with 175.7~260.5 nm in diameter. The images of FE-SEM revealed the porosity in 50.52~53.61% and pore area in 0.37~0.64 μm2 estimated by DiameterJ created for ImageJ. The qualitative antimicrobial analysis based on the inhibition zone test confirmed the antimicrobial activity and the quantitative analysis based on agar extraction process represented the antimicrobial death rate of 98.26~99.84% against Staphylococcus aureus (BCRC10781). The Ag/PU nonwoven mats showed good permeability and absorption according to moisture vapour transmission rate (MVRT) and water absorbency, respectively. In Ag ion release profile, we found that Ag ion reveals the burst release within 12 hours, showing maximum concentration at 0.69 μg/mL, and releases continuously up to 44 hours. Cytotoxicity assay showed that Ag/PU nonwoven mats reveal no significant cytoxicity toward fibroblast cell. LPS-induced macrophage test showed that Ag/PU nonwoven mats have anti-inflammatory ability. In vivo, full thickness skin removal (1.2×1.2 cm) was performed on the back of C57BL6/J mice in noninfected and infected animal models. Four groups of functional dressings were tested in this work including Aquacel, PU, 0.17M, and 0.34 M. After 10 days of treatment in infected animal models, Aquacel, PU, 0.17 M and 0.34 M were the closure area of the wound falling within 21.48%, 28.51%, 42.12% and 35.02%, respectively. After 10 days of treatment in fected animal models, Aquacel, PU, 0.17 M and 0.34 M were the closure area of the wound falling in infected animal models within 22.05%, 57.08%, 48.75% and 43.72%, respectively. Based on the histological analyses, the 0.17 M treated group outperformed all other groups in wound healing.

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