bR (bacteriorhodopsin) 為一個具有光驅動質子唧筒功能的蛋白質，存在於 Halobacterium halobium 的紫色細胞膜 (purple membrane, PM)內。本文主要的研究目的在於建立一個電化學光電系統，以量測PM溶液受光激發後所引起的電流變化。實驗中先由基本的比色管光電化學系統進行探討，以尋求系統的最適化操作條件與參數；而後再將此條件應用到 PDMS 陣列系統，並比較此二系統之差異。由初步的比色管實驗，發現工作電極以ITO玻璃、逆電極以白金棒、導電介質為銅片時最為適當，其光電流訊號值在微安培 (μA) 的範圍內；而將相同的操作條件應用到PDMS陣列系統也同樣可行。文中並就光電流訊號產生的機制進行探討。對比色管系統進一步探討，發現對系統最適合的PM 酸鹼值為 6；而電解液之最適值則是 7；且在不同酸鹼值下 PM 細胞膜的外觀有所變化。同時在固定 PM 溶液量下， PM 的濃度與燈源瓦數不會影響光電流訊號的大小；照光頻率越高，光電流訊號值會越小；而藍色與黃色濾片所測得之光電流訊號會較綠色及紅色濾片為大。以比色管與 PDMS 陣列系統做比較可以發現，PM體積的大小以及電極與銅片間的距離皆會影響光電流訊號的大小；且在相近的 PM 用量下由於系統尺寸的縮小，PDMS 陣列系統可較比色管系統測得較大的光電流訊號值。此光電化學系統的建立對於 bR 感光材料的應用上，樹立相當程度的研發基礎。

Bacteriorhodopsin (bR) is a protein of light-driven proton pump located in the purple membrane (PM) of Halobacterium halobium. In this study, two photo-electrochemical systems were set up to measure the photocurrent produced from illuminated PM. A cuvette system was first used to study the optimal operation condition including the intermediate, the pH values of PM and electrolyte solution, PM concentration, and the incident light intensity, which was subsequently applied to a PDMS array system. The cuvette study showed the highest photocurrent, which was in a range of microamperes, was achieved when ITO glass as the working electrode, a Pt wire as the counter electrode, and a Cu film as the intermediate were used. The production mechanism of the photocurrent was proposed according to its signal profile. The photocurrent of the PDMS array system was also obtained when the same electrodes/intermediate setup as that of the cuvette system was built. The studies on the cuvette system also revealed an optimal operation pH value of PM as 6, while the light intensity and the PM concentration did not have any effect on photocurrents if the same PM amount was used. The comparison between the cuvette and PDMS systems suggested that the distances between the electrodes and the Cu intermediate film determined the photocurrent values, yielding a higher signal in the latter system than in the former one if the same PM amount was used. This study provides valuable informations for the future photoelectric applications of the photosensitive bR.

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