研究生: |
廖清德 Ching-Te Liao |
---|---|
論文名稱: |
紫膜生物光電晶片於朝鮮薊抑菌研究之應用 Study of the antibacterial effect of artichoke extract using a purple membrane-based photoelectric biochip |
指導教授: |
陳秀美
Hsiu-Mei Chen |
口試委員: |
葉旻鑫
楊顓丞 |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2021 |
畢業學年度: | 109 |
語文別: | 中文 |
論文頁數: | 87 |
中文關鍵詞: | 紫膜 、抑菌 、朝鮮薊 、光電晶片 |
外文關鍵詞: | Purple membrane, Antibacterial, artichoke, photoelectric chip |
相關次數: | 點閱:184 下載:0 |
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本論文利用以Halobacterium salinarum紫膜 (purple membrane,PM)為訊號轉換器的生物光電感測器對於朝鮮薊萃取液的抑菌效果進行檢測與探討。由於微生物的遮光性可降低塗覆有PM膜電極晶片的光電流產生強度,因此我們可量化觀察朝鮮薊萃取液添加對微生物的增生或成膜之影響。本論文分為兩個部分,第一部分使用可進行即時檢測之連續注流式PM膜雙層微流道感測器系統,將塗覆有微生物的玻璃置於上層微流道,並連續注入添加不同稀釋比例朝鮮薊萃取液添加量之培養基,同時對置於下層微流道的PM膜電極晶片連續進行光電流量測,如此可即時量化偵測生物膜的生長情形。結果發現,於40 μL/min流速下,流動培養基中添加15倍稀釋之朝鮮薊萃取液時,有最佳的抑菌效果;其中對於Candida albicans、Candida parapsilosis與Escherichia coli的抑制效果最為顯著。第二部分使用可分別檢測真菌、格蘭氏陽性菌與格蘭氏陰性菌的三種PM膜微生物感測晶片,對於靜態培養時添加15倍與25倍兩種不同稀釋比例朝鮮薊萃取液的微生物溶液進行不同培養時間下菌濃度量化檢測。結果同樣顯示培養時添加15倍稀釋之朝鮮薊萃取液有最佳的抑菌效果。抑菌效果於第12小時培養時達顛峰,可維持18小時;但於第24小時即失去效果。本研究顯示朝鮮薊萃取液有明確抑菌效果,且PM膜光電感測器適合應用於微生物生長與培養,以及材料或物質的抑菌性研究。
We use a biophotoelectric sensor with Halobacterium salinarum (purple membrane ,PM) as the signal transducer to detect and discuss the antibacterial effect of artichoke extract. Since shading the microorganisms can reduce the intensity of the photocurrent generated by the chip, we can quantify the effect of the addition of artichoke extract on the microorganisms. This paper is divided into two parts. The first part uses a continuous injection PM membrane double-layer microfluidic sensor system that can be used for real-time detection. The glass coated with microorganisms is placed in the upper microfluidic channel and continuously injected Different dilution ratios of artichoke extracts are added to the medium, while continuous photoelectric flow measurement is performed on the PM chips placed in the lower microchannel, so that the growth of biofilm can be quantitatively detected in immediate time. The results showed that, at a flow rate of 40 μL/min, when 15 times diluted artichoke extract was added to the flowing medium, the best antibacterial effect was found; Artichoke extract has the most significant inhibitory effect on Candida albicans, Candida parapsilosis and Escherichia coli. The second part uses three types of PM chips that can detect fungi, Gram-positive bacteria and Gram-negative bacteria, for microorganisms with two different dilution ratios of artichoke extract, 15 times and 25 times, added during static culture The solution was subjected to quantitative detection of bacterial concentration under different times. The results also showed that adding 15 times diluted artichoke extract has the best antibacterial effect. The antibacterial effect reached its peak at the 12th hour of culture and could be maintained for 18 hours; however, the effect was lost at the 24th hour. This study shows that the artichoke extract has a clear antibacterial effect, and the PM photoelectric sensor is suitable for microbial growth and cultivation, as well as the antibacterial research of materials or substances.
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