研究生: |
周啟君 Chi-chun Chou |
---|---|
論文名稱: |
製備與評估適合作為抗菌組織調理材之聚甲基丙烯酸乙酯之微米顆粒 Preparation and Characterizations of Various poly (ethyl methacrylate) Microparticles Intended for a Novel Anti-fungal Tissue Conditioner |
指導教授: |
高震宇
Chen yu-Kao |
口試委員: |
蔡協致
none 李曉屏 none 朱兆秀 none |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 醫學工程研究所 Graduate Institute of Biomedical Engineering |
論文出版年: | 2013 |
畢業學年度: | 101 |
語文別: | 中文 |
論文頁數: | 70 |
中文關鍵詞: | 組織調理材 、顆粒大小 、表面性質 、凝膠時間 、伊曲康唑 、抗菌性 |
外文關鍵詞: | tissue conditioner, particle size, surface properties, gelation time, itraconazole, antibacterial |
相關次數: | 點閱:248 下載:2 |
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臨床上組織調理材 (tissue conditioner) 常被用於治療義齒性口腔炎 (denture-related stomatitis) 之初期發炎反應。其中組織調理材粉劑 (powder)主要由聚甲基丙烯酸乙酯 poly(ethyl methacrylate) (PEMA) 及其共聚物之微粒 (microparticles) 所組成。然而粉劑微粒的顆粒大小、形態、表面性質及包裹藥物皆會影響組織調理材的機械性質與凝膠時間 (gelation time),進而影響其臨床應用性,並且能達到抑菌效果。
本研究目的為發展具有抑菌能力之組織調理材,藉由不同之製備條件與分析技術,製備所需之含抗真菌藥物伊曲康唑 itraconazole 之PEMA微粒。此微粒所製備的組織調理材則以高效能液相層析 (HPLC) 量測 itraconazole 之藥物釋放模式,及利用體外抑制白色念珠菌 (Candida albican) 抑菌實驗評估其抗菌效果。
初步研究顯示,粒徑介於23.98 - 19.10 μm 之 PEMA 含藥微粒(含3.5 %之 itraconazole) 之凝膠時間為 5.28 ± 1.68 min,與市售組織調理材最為接近。而此微粒所製備之含藥藥物之組織調理材可在 14 天內持續釋放 itraconazole,且白色念珠菌 (Candida albicans) (BCRC 21538) 之體外抑菌實驗也同時證實此含藥組織調理材可維持長效性抑菌能力達14 天。
Poly(ethyl methacrylate) (PEMA) and it’s copolymers are the main powder component of the tissue conditioner., a material used in the treatment of the denture-related stomatitis. Several things might affect the mechanical properties and gelation time of the tissue conditioners, including the particle size and surface properties of the PEMA particles, and adding of therapeutic agents to the tissue conditioners. In this study, various blank PEMA microparticles and intraconazole loaded PEMA microparticles were prepared by the emulsion technique. And the effect of particle size, surface morphology and properties of PEMA microparticles on its gelation time was investigated. In our study, the itraconazole-PEMA particles size range from 23.98 to 19.10 μm and are used to prepare the drug loaded tissue conditioners. And the gelation time of the drug loaded tissue conditioner is 5.28 ± 1.68 min which is the closest to the DENTSPLY Visco gel, a comericial tissue conditioner. More importantly, this drug loaded tissue conditioner could continuously release itraconazole and maintain its inhibitory effects of Candida albicans (BCRC 21538) for at least 14 days.
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