本研究主要為使用靜電紡絲技術製備聚乙烯醇(PVA)串珠奈米纖維複合膜應用於拋棄式網版印刷電極，利用串珠纖維的多孔性及高比表面積特性，相較於一般靜電紡絲奈米纖維具有更高的比表面積，並證實降低酵素的用量奈米串珠纖維複合膜依然具有一定的靈敏度，且可使用網版印刷電極進行電化學分析。此外除了透過電化學的計時安培法觀察靈敏度、抗干擾性、穩定性等方面進行分析以外。本研究也對奈米串珠纖維膜進行SEM與TEM結構微觀分析。此外為了進一步提高奈米串珠纖維複合膜的靈敏度，額外添加少許奈米材料如奈米金與氧化石墨烯，其中奈米金可增加導電度與減少應答時間，而氧化石墨烯則可將酵素的活性中心體拉於表面，進而提高靈敏度。此外本研究亦使用戊二醛交聯使酵素與聚乙烯醇共價鍵結，與使用試劑EDC與NHS將酵素接枝於氧化石墨烯製成試片，進行電化學分析。此外為了降低干擾，本研究也添加赤血鹽作為酵素反應時的電子傳媒，以降低電化學測試時的電壓施加量，使干擾物不會因電壓而反應，藉此達到抗干擾效果。
本研究成功製備出電紡聚乙烯醇/葡萄糖氧化酵素/赤血鹽奈米串珠纖維膜，此纖維膜的靈敏度高於一般電紡纖維薄膜約3.2倍，並且降低酵素用量40%後，依然擁有高於一般電紡纖維薄膜約1.3倍的靈敏度。
本研究亦使用戊二醛交聯技術進行靈敏度測試以及比較穩定性分析，由數據可得知雖然戊二醛交聯後的靈敏度增加量並不高，大約增加1.5倍而已，但戊二醛交聯後的穩定性卻比未交聯的穩定性高很多，證實交聯可將酵素固定於PVA上使酵素能更加穩定。

This study is using electrospinning PVA prepared beaded nanofibrous composite membrane applied to disposable screen-printed electrode, the property of beaded Nanofibrous membrane are porosity and high surface area, it has higher surface area compared with electrospinning nanofibrous membrane, and prove that decreased the amount of enzyme in the beaded nanofibrous membrane still have some sensitivity, and the beaded nanofibrous membrane can also use screen-printing electrode for electrochemical analysis.
Except observing sensitivity, interference resistance, and stability through chronoamperometry analysis, also observing beaded nanofibrous structure through scanning electron microscope (SEM) and transmission electron microscope (TEM). Moreover, to improve sensitivity of beaded nanofibrous membrane, adding small amount of grapnene oxide and nano gold particle, which nano gold can decrease the responsing time and increase the conductivity, and graphene oxide can make the active center more cross the surface of the enzyme. And we also use cross-linking method with glutaraldehyde and sulfuric acid, and using EDC & NHS to combine enzyme with graphene oxide, which can increase the sensitivity of the biosensor.
This study successful prepared PVA/Glucose oxidase/Ferricyanide salt beaded nanofibrous membrane, and this membrane can offer about 3.2 times of sensitivity than traditional electrospun nanofibrous film, and when deceasing about 40% of enzyme usage amount, it still have about 1.3 times of sensitivity than traditional electrospun nanofibrous film.
Using cross-linking method can increase about 1.5times of the sensitivity, it is not a huge increase, but after cross-linking can enhance the stability of the biosensor.

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