本研究旨在開發用於骨組織工程之原位凝膠支架，以恢復骨組織之功能。為達此目標，此一凝膠係由F127及幾丁聚醣構成。所得之溫度可逆性凝膠以流變儀測定其凝膠溫度(Tgel)、黏彈性質、黏膜黏著力，以便最佳化凝膠之組成。在特定比例下，該凝膠具有接近體溫之Tgel。添加幾丁聚醣不影響F127的自組裝特性，僅會提升其Tgel。此外，幾丁聚醣會強化凝膠的力學性質，可能係由於幾丁聚醣與F127微胞間有氫鍵作用而強化其結構。DSC結果顯示幾丁聚醣影響了水分子與F127之作用力。TGA的結果亦支持水分子與凝膠之相互作用。黏膜黏著實驗顯示F127/幾丁聚醣與黏液素(mucin)有流變學相乘效應，使其流動行為由牛頓流體轉變成為擬塑性。藥物釋放實驗顯示F127/幾丁聚醣凝膠可延緩羥乙氧甲鳥嘌呤(acyclovir)之釋放達20分鐘以上。此外，以細胞增生性、細胞貼附性、全血測試法證實F127/幾丁聚醣凝膠具有生物相容性。

This work is to develop an in situ gelation scaffold for bone tissue engineering to restore and maintain the function of human bone tissues. To meet this goal, the gel is comprised of F127 (a poloxamer) and chitosan. The gelation temperature (Tgel), viscoelastic properties and mucoadhesive force of the systems were investigated and optimized by means of rheological analyses. At specific concentrations, the resulting thermoreversible gel exhibited a Tgel close to the body temperature. The addition of chitosan did not hamper the self assembling process of F127, only elevating the gelation temperature. Furthermore, chitosan can improve the rheological properties of the gel, possibly due to the interaction between chitosan and F127 micelles through hydrogen bond, which reinforced the gel structure. The DSC results showed the interactions between water and F127 gels, indicating the influence of chitosan on the physicochemical features of gels. TGA results also indicated the interactions between the gels and water. Mucoadhesion experiments showed a rheological synergism between F127/chitosan gels and mucin dispersion, changing the flow behavior from a Newtonian fluid to a pseudoplastic fluid. In vitro release experiments indicated that the optimized gel was able to prolong and control acyclovir release for more than 20 min. Biocompatibility of the gel of F127 and chitosan was observed based on cell proliferation, protein adsorption and complete blood count test.

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