現今，微氣泡已廣泛被應用在醫學上，無論是顯影劑用於醫學診斷，又或是使微氣泡產生穴蝕效應，作為載體釋放藥物的方式。其相關研究儀器設備、藥物都價值不斐，本研究的目的在於用簡單、低成本的方法製備含微氣泡的靛氰綠-紫草素海藻酸鹽載體，其中靛氰綠是醫學上常用的螢光顯影劑，而紫草素在許多文獻中被指出具有抗癌的效果。本研究採用機械性攪拌方式製備出微氣泡，並利用電噴灑裝置使海藻膠與鈣離子產生共交聯，將疏水性藥物、微氣泡包覆在載體中。本研究採用超音波震盪、往復式震盪恆溫水槽兩種釋放模式，進行體外藥物釋放曲線圖的比較，再對SKOV3、CP70(/A2780)兩株卵巢癌細胞進行細胞毒性測試。針對本研究製備載體之應用，分別拍攝了超音波影像及在紅外光下的螢光影像，結合兩種不同顯影功能的顯影劑。實驗結果發現靛氰綠可以做為界面活性劑增加母液的起泡性，而與往復式震盪恆溫水槽相比，微氣泡透過超音波震盪的方式，可以在固定的時間內釋放出更多藥物。從細胞毒性測試的結果圖中也可以說明紫草素似乎對CP70(/A2780)細胞更有毒性效果。

Nowadays, microbubbles have been widely used in medical science, such as contrast agent for medical diagnosis, or bursting microbubbles to release the drug from the carrier. As mentioned above, the equipment and drugs for those experiment are expensive. The purpose of this research is to prepare indocyanine green-shikonin alginate microbubbles by a simple and low-cost method. By the way, indocyanine green is a commonly fluorescent agent in medicine, and shikonin has demonstrated potent anti-cancer activities. In this study, microbubbles were produced by stirring in a high speed. The calcium ions in the solution cross-linked alginate during the electrospray, then the hydrophobic drug and microbubbles would be encapsulated in the carrier. The study was compared the curve of drug release in two models, which one was in ultrasonic cleaner, and the other one was in reciprocal shaking water baths. In order to prove the cytotoxicity of ovarian cancer cells, we chose SKOV3 and CP70 (/A2780) cells to do the test. Then, ultrasonic images and fluorescent images were taken respectively as their applications. The experimental results were found that indocyanine green has amphiphilic properties in chemical structure, so it’s a surfactant to increase the foaming property of solution. In a fixed time, More drug is released by ultrasonication. The result of MTT assay seems that shikonin is more toxic on CP70(/A2780) cells.

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