微生物燃料電池可分為單室和雙室，單室微生物燃料電池省去了陰極室，有著低內組等優點，因為利用氧氣作為陰極的氧化劑，所以讓電池沒有氧化劑消耗的問題。
3D 列印是以積層製造 (Additive Manufacturing) 為主的一種技術，透過 3D 列印可將微生物燃料電池的結構進一步縮小，此製程更加能夠節省製造時間及成本，對於實驗會快速且方便許多。
微型燃料電池因為電極表面積非常小導致電力輸出不佳，在電極表面積接觸面積非常小之狀況下，電極材料的高導電率、高表面積、
無腐蝕性等等性質就顯得相當重要，微型微生物燃料電池因為在陽極室會產生二氧化碳氣泡降低有效電極面積，本實驗在電池上增加孔洞設計來排出電池內的氣泡，增加其電池的放電能力。本研究利用碳布作為空氣陰極，一開始探討不同碳材塗佈於碳布上對於電池發電的影響，塗佈奈米碳管得到了最好的結果，在碳布上塗佈奈米碳管得到了最大的電流密度值3.889A/m2及最大功率密度值0.385W/m2，在觸媒比較實驗B12/CNT得到了最好的結果，提升了開路電位值 0.667V，提升了最大功率密度0.57W/m2。

There are two types of microbial fuel cells: single chamber and double chamber. Single chamber microbial fuel cell does not have cathode chamber. In this research, the proton exchange membrane was also removed, so it has low resistance characteristics. Because oxygen was served as the oxidant of cathode, battery does not have the problem of oxidant consumption.
Combining 3D printing with research is very popular nowadays. This technology is mainly based on additive manufacturing. Through 3D printing, the structure of microbial fuel cells can be further reduced, saving both time and money.
Consider the surface area of the electrodes of micro fuel cells is very small, the output power is quite low as well. When contact area of the electrodes is very small, electrode materials’ high electric conductivity, large surface area, and incorrosiveness are relatively important. Anode chamber of micro-bio-fuel cell will produce bubbles of carbon dioxide, which would reduce the effective electrode area. In this research, a hole within the battery structure was design to exhale bubbles inside the battery to increase the discharge capacity of battery. In this experiment, carbon cloth is used as an air cathode for comparison of different carbon materials. Finally, carbon cloth coated with carbon nanotubes (CNT) had obtained the best result. The maximum current densities were 3.889 A/m2 and 0.385 A/m2, which were 211% and 243% higher than those with the carbon cloth electrodes without modification.
In the catalyst comparison, B12/ CNT had the best result. Their open circuit potential (OCV) were 0.667V and the maximum power density were 0.57 W/m2, which were 99% and 204% higher than that of carbon cloth electrodes without catalyst.

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