血液凝血檢測分析仍是目前外科手術、心血管疾病、慢性病併發症及血友病的重要依據。傳統檢測血液凝血酶原時間，一般使用自動凝血分析儀來做檢測，但是儀器設備系統昂貴、體積龐大、成本較高且檢測時間過長。近年來，SPR干涉系統在生物醫學的檢測上面，逐漸取代傳統的方法，SPR更是被廣泛的應用在生物檢測的範圍內，因為它有著對不同折射率的待測物高靈敏度的特性。其中，頻譜干涉表面電漿共振的原理，就是當光源入射經過一極化分光器，使入射之P極化與S極化光強度相同。光在進入稜鏡後會使P極化與S極化之光之間產生一延遲。由於只有P極化之光會產生SPR現象，於是在稜鏡內P極化與S極化光之間又產生了一延遲，接著經由稜鏡後方的線性偏振片結合而干涉，再由光頻譜分析儀(OSA)接收，直接觀察其干涉結果。
在本論文中，我們利用頻譜干涉表面電漿共振感測器之生醫檢測進行凝血時間的量測，將凝血因子依序與5mM、25mM、50mM、150mM、200mM之CaCl2 溶液配置，並以OSA(Optical Spectrum Analyzer)記錄干涉頻譜，量測出相對應的凝血時間為364秒至260秒逐漸縮短。並且當CaCl2溶液每增加1mM時，所需的時間將減少0.56秒。本系統血液樣本需求量較少且血液樣本可使用全血樣本，而非接觸式檢測，如此一來將減少對凝血機制之影響，對生醫領域提供一快速、方便簡單及低成本的光學生物檢測技術。

The blood coagulation characterization is important for surgery, cardiovascular disease, chronic disease complications and hemophilia. It is traditionally to use the automatic coagulation analyzer to detect the blood prothrombin time, but it possesses high price, bulky size, and long detection time. In recent years, surface plasmon resonance (SPR) interference systems own the potential in high sensitivity. The principle of interferometry based SPR is that when the light source is incident to a polarization beam splitter, the incident P-polarized and S-polarized light are adjusted to the approximately equal intensity. When these two polarizations enter the SPR sensor, there is a delay between the P and S-polarized light due to the P-polarized light producing SPR phenomenon. Then a delay will be formed between the P- and S-polarized lights to form the interference after the linear polarizer. Then the optical spectrum analyzer (OSA) can be utilized to directly observe interferograms.
In this thesis, we use the spectral interferometry-based SPR to biosense blood coagulation for coagulation time. After mixing the blood with CaCl2 with the concentrations of 5 mM, 25 mM, 50 mM, 150 mM and 200 mM, the coagulation time demonstrates from 364 seconds to 260 seconds. When CaCl2 increases by 1 mM, the coagulation time will be reduced by 0.56 seconds. The shortest time we could characterize is 260 seconds, and the solution is 200 mM CaCl2 due to the system limitation. In conclusion, the SPR can provide a fast, convenient and low-cost optical bio-detection technology for blood coagulation.

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