在本研究中，吾人使用微影技術(Photolithography)於矽晶片上製備同心圓狀微米級溝槽(microgrooves)，其溝槽寬度由圓心(約20贡um)往邊緣(約1贡um)遞減，其溝槽深度則維持在轨贡1 um左右。於此一矽晶片上進行大鼠骨瘤細胞(ROS)的培養，接觸引導(Contact guidance)的現象清晰可見，且細胞之貼附數目將隨溝槽寬度的增加而下降，意即最高之細胞貼附密度發生在具有最小溝槽寬度的位置，此外，較窄的溝槽同樣有效地促進了細胞的增生。

吾人接著將微米溝槽由矽晶圓複製至PDMS基材上，並以氬氣電漿調整其親疏水性。實驗結果指出在親水性的PDMS基材上，細胞排列之方向性、細胞貼附與細胞增生的情形皆較於疏水性PDMS基材上之細胞為佳，這主要是由於表面親水性的增加提高了基材本身對細胞的親和性，也因如此，細胞能偵測到表面微結構的差異，故細胞的排列、貼附與增生皆會隨著溝槽寬度的減少而提升。相反地，在水性的PDMS基材上，由於其與細胞親和性差，故無法發現溝槽的結構或存在對細胞的數量與方向性產生顯著的影響。在細胞分化方面，吾人亦觀察到在親水性PDMS基材上的細胞更傾向於分化成骨組織，此點可由其上之骨細胞表現出較明顯的骨分化指標而證明。此外，降低溝槽寬度亦能有效促進骨蛋白與細胞鈣化的表現，以上的現象可能與蛋白質在表面上的沉積狀況有關。

最後，吾人使用臭氧活化方式將RGD胜肽片段接枝於有溝槽結構的PDMS基材上，與未改質之樣品相較，雖然兩者之親水性相近，但RGD片段確實促進了細胞的貼附，且所培養之細胞也能偵測到基材表面之結構狀態，也就是說，溝槽寬度之降低能成功提昇細胞的貼附、增生、排列與骨分化。由以上之結果可發現，藉由結合材料表面的物理結構與化學性質，骨類細胞的生長與行為可被成功控制。

Photolithography methods are used in this thesis to create microgroove pattern on Si wafer. The Si wafer has characteristics of continuous groove width varied from 1 to 20μm, while the depth was kept as 1μm. ROS cells were cultured onto Si wafer surfaces. “Contact guidance” phenomena were found on the microgrooved substrates. Increasing the groove width would result in the lower cell attachment and cell density. Microgroove pattern was successfully replicated from Si wafer to PDMS substrates. The groove depth was decreased to 0.8 μm. Ar plasma treatment was used to create hydrophilic PDMS. The ROS cell observation was conducted both on hydrophobic and hydrophilic PDMS. The cell alignment on the hydrophilic PDMS was significantly higher than on hydrophobic samples. Hydrophilic PDMS could enhance the cells attachment and proliferation, due to its high affinity to the cells, and the cells were able to sense the topographical cues and affect their proliferation. Decreasing the groove width would result in a higher cell proliferation and bone mineralization, with the highest expression on the smallest groove width. On hydrophobic PDMS, the cell affinity was poor, and the cells could not recognize the topographical cues, hence the variation in groove width was not efficient on the cell proliferation. RGD peptide was also grafted on PDMS surfaces via ozone-induced grafting process. Even though the RGD-modified PDMS was hydrophobic, however, the topographical cues still could be sense by the cells. The combination of topographical and chemical cues could enhance the cell attachment and determined the cell behavior and final cellular mineralization as well.

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